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Science 15 June 2007:
Vol. 316. no. 5831, pp. 1625 - 1628
DOI: 10.1126/science.1139816
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Reports

Sequence Finishing and Mapping of Drosophila melanogaster Heterochromatin

Roger A. Hoskins,1* Joseph W. Carlson,1* Cameron Kennedy,1 David Acevedo,1 Martha Evans-Holm,1 Erwin Frise,1 Kenneth H. Wan,1 Soo Park,1 Maria Mendez-Lago,2 Fabrizio Rossi,3 Alfredo Villasante,2 Patrizio Dimitri,3 Gary H. Karpen,1,4 Susan E. Celniker1{dagger}

Genome sequences for most metazoans and plants are incomplete because of the presence of repeated DNA in the heterochromatin. The heterochromatic regions of Drosophila melanogaster contain 20 million bases (Mb) of sequence amenable to mapping, sequence assembly, and finishing. We describe the generation of 15 Mb of finished or improved heterochromatic sequence with the use of available clone resources and assembly methods. We also constructed a bacterial artificial chromosome–based physical map that spans 13 Mb of the pericentromeric heterochromatin and a cytogenetic map that positions 11 Mb in specific chromosomal locations. We have approached a complete assembly and mapping of the nonsatellite component of Drosophila heterochromatin. The strategy we describe is also applicable to generating substantially more information about heterochromatin in other species, including humans.

1 Department of Genome and Computational Biology, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
2 Centro de Biologia Molecular Severo Ochoa, CSIC-UAM, Cantoblanco 28049, Madrid, Spain.
3 Dipartimento di Genetica e Biologia Molecolare "Charles Darwin," Universita "La Sapienza," 00185 Roma, Italy.
4 Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.

* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: celniker{at}fruitfly.org

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