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Science 15 June 2007:
Vol. 316. no. 5831, pp. 1586 - 1591
DOI: 10.1126/science.1139815
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Research Articles

The Release 5.1 Annotation of Drosophila melanogaster Heterochromatin

Christopher D. Smith,1,2 ShengQiang Shu,3 Christopher J. Mungall,3 Gary H. Karpen2,4*

The repetitive DNA that constitutes most of the heterochromatic regions of metazoan genomes has hindered the comprehensive analysis of gene content and other functions. We have generated a detailed computational and manual annotation of 24 megabases of heterochromatic sequence in the Release 5 Drosophila melanogaster genome sequence. The heterochromatin contains a minimum of 230 to 254 protein-coding genes, which are conserved in other Drosophilids and more diverged species, as well as 32 pseudogenes and 13 noncoding RNAs. Improved methods revealed that more than 77% of this heterochromatin sequence, including introns and intergenic regions, is composed of fragmented and nested transposable elements and other repeated DNAs. Drosophila heterochromatin contains "islands" of highly conserved genes embedded in these "oceans" of complex repeats, which may require special expression and splicing mechanisms.

1 Department of Biology, San Francisco State University, San Francisco, CA 94132, USA.
2 Drosophila Heterochromatin Genome Project, Department of Genome and Computational Biology, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
3 National Center for Biomedical Ontology, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
4 Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA.

* To whom correspondence should be addressed. E-mail: karpen{at}fruitfly.org

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Polytene Chromosomal Maps of 11 Drosophila Species: The Order of Genomic Scaffolds Inferred From Genetic and Physical Maps.
S. W. Schaeffer, A. Bhutkar, B. F. McAllister, M. Matsuda, L. M. Matzkin, P. M. O'Grady, C. Rohde, V. L. S. Valente, M. Aguade, W. W. Anderson, et al. (2008)
Genetics 179, 1601-1655
   Abstract »    Full Text »    PDF »
An Investigation of Heterochromatin Domains on the Fourth Chromosome of Drosophila melanogaster.
N. C. Riddle, W. Leung, K. A. Haynes, H. Granok, J. Wuller, and S. C. R. Elgin (2008)
Genetics 178, 1177-1191
   Abstract »    Full Text »    PDF »
Polymorphic Y Chromosomes Harbor Cryptic Variation with Manifold Functional Consequences.
B. Lemos, L. O. Araripe, and D. L. Hartl (2008)
Science 319, 91-93
   Abstract »    Full Text »    PDF »
A portrait of copy-number polymorphism in Drosophila melanogaster.
E. B. Dopman and D. L. Hartl (2007)
PNAS 104, 19920-19925
   Abstract »    Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)