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ReportsRevisiting the Role of the Mother Centriole in Centriole BiogenesisCentrioles duplicate once in each cell division cycle through so-called templated or canonical duplication. SAK, also called PLK4 (SAK/PLK4), a kinase implicated in tumor development, is an upstream regulator of canonical biogenesis necessary for centriole formation. We found that overexpression of SAK/PLK4 could induce amplification of centrioles in Drosophila embryos and their de novo formation in unfertilized eggs. Both processes required the activity of DSAS-6 and DSAS-4, two molecules required for canonical duplication. Thus, centriole biogenesis is a template-free self-assembly process triggered and regulated by molecules that ordinarily associate with the existing centriole. The mother centriole is not a bona fide template but a platform for a set of regulatory molecules that catalyzes and regulates daughter centriole assembly.
1 Instituto Gulbenkian de Ciência, Cell Cycle Regulation Laboratory, Rua da Quinta Grande, 6, P-2780-156 Oeiras, Portugal. * To whom correspondence should be addressed. E-mail: mdias{at}igc.gulbenkian.pt (M.B.-D.); dmg25{at}hermes.cam.ac.uk (D.M.G.)
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Science. ISSN 0036-8075 (print), 1095-9203 (online)