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Originally published in Science Express on 26 April 2007
Science 18 May 2007:
Vol. 316. no. 5827, pp. 1046 - 1050
DOI: 10.1126/science.1142950
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Reports

Revisiting the Role of the Mother Centriole in Centriole Biogenesis

A. Rodrigues-Martins,1,2 M. Riparbelli,3 G. Callaini,3 D. M. Glover,2* M. Bettencourt-Dias1,2*

Centrioles duplicate once in each cell division cycle through so-called templated or canonical duplication. SAK, also called PLK4 (SAK/PLK4), a kinase implicated in tumor development, is an upstream regulator of canonical biogenesis necessary for centriole formation. We found that overexpression of SAK/PLK4 could induce amplification of centrioles in Drosophila embryos and their de novo formation in unfertilized eggs. Both processes required the activity of DSAS-6 and DSAS-4, two molecules required for canonical duplication. Thus, centriole biogenesis is a template-free self-assembly process triggered and regulated by molecules that ordinarily associate with the existing centriole. The mother centriole is not a bona fide template but a platform for a set of regulatory molecules that catalyzes and regulates daughter centriole assembly.

1 Instituto Gulbenkian de Ciência, Cell Cycle Regulation Laboratory, Rua da Quinta Grande, 6, P-2780-156 Oeiras, Portugal.
2 Cancer Research UK, Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK.
3 Department of Evolutionary Biology, University of Siena, Via A. Moro 4, I-53100 Siena, Italy.

* To whom correspondence should be addressed. E-mail: mdias{at}igc.gulbenkian.pt (M.B.-D.); dmg25{at}hermes.cam.ac.uk (D.M.G.)

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
ECRG2 Disruption Leads to Centrosome Amplification and Spindle Checkpoint Defects Contributing Chromosome Instability.
X. Cheng, Z. Shen, J. Yang, S.-H. Lu, and Y. Cui (2008)
J. Biol. Chem. 283, 5888-5898
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SAS-4 is recruited to a dynamic structure in newly forming centrioles that is stabilized by the {gamma}-tubulin-mediated addition of centriolar microtubules.
A. Dammermann, P. S. Maddox, A. Desai, and K. Oegema (2008)
J. Cell Biol. 180, 771-785
   Abstract »    Full Text »    PDF »
The Golgi Protein GM130 Regulates Centrosome Morphology and Function.
A. Kodani and C. Sutterlin (2008)
Mol. Biol. Cell 19, 745-753
   Abstract »    Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)