Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Originally published in Science Express on 26 April 2007
Science 18 May 2007:
Vol. 316. no. 5827, pp. 1046 - 1050
DOI: 10.1126/science.1142950
Prev | Table of Contents | Next

Reports

Revisiting the Role of the Mother Centriole in Centriole Biogenesis

A. Rodrigues-Martins,1,2 M. Riparbelli,3 G. Callaini,3 D. M. Glover,2* M. Bettencourt-Dias1,2*

Centrioles duplicate once in each cell division cycle through so-called templated or canonical duplication. SAK, also called PLK4 (SAK/PLK4), a kinase implicated in tumor development, is an upstream regulator of canonical biogenesis necessary for centriole formation. We found that overexpression of SAK/PLK4 could induce amplification of centrioles in Drosophila embryos and their de novo formation in unfertilized eggs. Both processes required the activity of DSAS-6 and DSAS-4, two molecules required for canonical duplication. Thus, centriole biogenesis is a template-free self-assembly process triggered and regulated by molecules that ordinarily associate with the existing centriole. The mother centriole is not a bona fide template but a platform for a set of regulatory molecules that catalyzes and regulates daughter centriole assembly.

1 Instituto Gulbenkian de Ciência, Cell Cycle Regulation Laboratory, Rua da Quinta Grande, 6, P-2780-156 Oeiras, Portugal.
2 Cancer Research UK, Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK.
3 Department of Evolutionary Biology, University of Siena, Via A. Moro 4, I-53100 Siena, Italy.

* To whom correspondence should be addressed. E-mail: mdias{at}igc.gulbenkian.pt (M.B.-D.); dmg25{at}hermes.cam.ac.uk (D.M.G.)

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Ana3 is a conserved protein required for the structural integrity of centrioles and basal bodies.
N. R. Stevens, J. Dobbelaere, A. Wainman, F. Gergely, and J. W. Raff (2009)
J. Cell Biol. 187, 355-363
   Abstract »    Full Text »    PDF »
Ciliary and centrosomal defects associated with mutation and depletion of the Meckel syndrome genes MKS1 and MKS3.
R. Tammachote, C. J. Hommerding, R. M. Sinders, C. A. Miller, P. G. Czarnecki, A. C. Leightner, J. L. Salisbury, C. J. Ward, V. E. Torres, V. H. Gattone II, et al. (2009)
Hum. Mol. Genet. 18, 3311-3323
   Abstract »    Full Text »    PDF »
Microtubule Cytoskeleton Remodeling by Acentriolar Microtubule-organizing Centers at the Entry and Exit from Mitosis in Drosophila Somatic Cells.
S. Moutinho-Pereira, A. Debec, and H. Maiato (2009)
Mol. Biol. Cell 20, 2796-2808
   Abstract »    Full Text »    PDF »
The Two SAS-6 Homologs in Tetrahymena thermophila Have Distinct Functions in Basal Body Assembly.
B. P. Culver, J. B. Meehl, T. H. Giddings Jr, and M. Winey (2009)
Mol. Biol. Cell 20, 1865-1877
   Abstract »    Full Text »    PDF »
The SCFSlimb ubiquitin ligase regulates Plk4/Sak levels to block centriole reduplication.
G. C. Rogers, N. M. Rusan, D. M. Roberts, M. Peifer, and S. L. Rogers (2009)
J. Cell Biol. 184, 225-239
   Abstract »    Full Text »    PDF »
Drosophila asterless and Vertebrate Cep152 Are Orthologs Essential for Centriole Duplication.
S. Blachon, J. Gopalakrishnan, Y. Omori, A. Polyanovsky, A. Church, D. Nicastro, J. Malicki, and T. Avidor-Reiss (2008)
Genetics 180, 2081-2094
   Abstract »    Full Text »    PDF »
ECRG2 Disruption Leads to Centrosome Amplification and Spindle Checkpoint Defects Contributing Chromosome Instability.
X. Cheng, Z. Shen, J. Yang, S.-H. Lu, and Y. Cui (2008)
J. Biol. Chem. 283, 5888-5898
   Abstract »    Full Text »    PDF »
SAS-4 is recruited to a dynamic structure in newly forming centrioles that is stabilized by the {gamma}-tubulin-mediated addition of centriolar microtubules.
A. Dammermann, P. S. Maddox, A. Desai, and K. Oegema (2008)
J. Cell Biol. 180, 771-785
   Abstract »    Full Text »    PDF »
The Golgi Protein GM130 Regulates Centrosome Morphology and Function.
A. Kodani and C. Sutterlin (2008)
Mol. Biol. Cell 19, 745-753
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)