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Requirement of bic/microRNA-155 for Normal Immune Function
Antony Rodriguez,1*Elena Vigorito,2*Simon Clare,1Madhuri V. Warren,1,3Philippe Couttet,1Dalya R. Soond,2Stijn van Dongen,1Russell J. Grocock,1Partha P. Das,4Eric A. Miska,4David Vetrie,1Klaus Okkenhaug,2Anton J. Enright,1Gordon Dougan,1Martin Turner,2Allan Bradley1
MicroRNAs are a class of small RNAs that are increasingly beingrecognized as important regulators of gene expression. Althoughhundreds of microRNAs are present in the mammalian genome, geneticstudies addressing their physiological roles are at an earlystage. We have shown that mice deficient for bic/microRNA-155are immunodeficient and display increased lung airway remodeling.We demonstrate a requirement of bic/microRNA-155 for the functionof B and T lymphocytes and dendritic cells. Transcriptome analysisof bic/microRNA-155deficient CD4+ T cells identifieda wide spectrum of microRNA-155regulated genes, includingcytokines, chemokines, and transcription factors. Our work suggeststhat bic/microRNA-155 plays a key role in the homeostasis andfunction of the immune system.
1 The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK. 2 Laboratory of Lymphocyte Signalling and Development, The Babraham Institute, Cambridge, CB2 4AT, UK. 3 Department of Pathology, Addenbroke's Hospital, University of Cambridge, Cambridge, CB2 2QQ, UK. 4 Gurdon Institute and Department of Biochemistry, University of Cambridge, Cambridge, CB2 1QN, UK.
* These authors contributed equally to this work
To whom correspondence should be addressed: abradley{at}sanger.ac.uk (A.B.); martin.turner{at}bbsrc.ac.uk (M.T.)
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