Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Originally published in Science Express on 5 April 2007
Science 20 April 2007:
Vol. 316. no. 5823, pp. 417 - 421
DOI: 10.1126/science.1141314
Prev | Table of Contents | Next

Research Articles

Genes Required for Mitotic Spindle Assembly in Drosophila S2 Cells

Gohta Goshima,1,3* Roy Wollman,2,3 Sarah S. Goodwin,1 Nan Zhang,1 Jonathan M. Scholey,2 Ronald D. Vale,1,3{dagger} Nico Stuurman1,3

The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spindle assembly, more than half of which were unexpected. The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes are positioned; and how centrioles, centrosomes, and kinetochores are assembled.

1 Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA.
2 Department of Molecular and Cellular Biology, University of California, Davis, One Shields Avenue, 3203 Life Sciences, Davis, CA 95616, USA.
3 Physiology Course, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543, USA.

* Present address: Institute for Advanced Research, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan.

{dagger} To whom correspondence should be addressed. E-mail: vale{at}cmp.ucsf.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Nek6 and Nek7 Protein Kinases Are Required for Robust Mitotic Spindle Formation and Cytokinesis.
L. O'Regan and A. M. Fry (2009)
Mol. Cell. Biol. 29, 3975-3990
   Abstract »    Full Text »    PDF »
Microtubule Cytoskeleton Remodeling by Acentriolar Microtubule-organizing Centers at the Entry and Exit from Mitosis in Drosophila Somatic Cells.
S. Moutinho-Pereira, A. Debec, and H. Maiato (2009)
Mol. Biol. Cell 20, 2796-2808
   Abstract »    Full Text »    PDF »
Drosophila Bld10 Is a Centriolar Protein That Regulates Centriole, Basal Body, and Motile Cilium Assembly.
V. Mottier-Pavie and T. L. Megraw (2009)
Mol. Biol. Cell 20, 2605-2614
   Abstract »    Full Text »    PDF »
A Proximal Centriole-Like Structure Is Present in Drosophila Spermatids and Can Serve as a Model to Study Centriole Duplication.
S. Blachon, X. Cai, K. A. Roberts, K. Yang, A. Polyanovsky, A. Church, and T. Avidor-Reiss (2009)
Genetics 182, 133-144
   Abstract »    Full Text »    PDF »
The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells.
R. Uehara, R.-s. Nozawa, A. Tomioka, S. Petry, R. D. Vale, C. Obuse, and G. Goshima (2009)
PNAS 106, 6998-7003
   Abstract »    Full Text »    PDF »
Core Exosome-independent Roles for Rrp6 in Cell Cycle Progression.
A. C. Graham, D. L. Kiss, and E. D. Andrulis (2009)
Mol. Biol. Cell 20, 2242-2253
   Abstract »    Full Text »    PDF »
Molecular Dissection of the Centrosome Overduplication Pathway in S-Phase-Arrested Cells.
S. L. Prosser, K. R. Straatman, and A. M. Fry (2009)
Mol. Cell. Biol. 29, 1760-1773
   Abstract »    Full Text »    PDF »
The Putative RNA-Processing Protein, THO2, is a Microtubule-Associated Protein in Tobacco.
T. Hamada, H. Igarashi, R. Taguchi, M. Fujiwara, Y. Fukao, T. Shimmen, E. Yokota, and S. Sonobe (2009)
Plant Cell Physiol. 50, 801-811
   Abstract »    Full Text »    PDF »
Wac: a new Augmin subunit required for chromosome alignment but not for acentrosomal microtubule assembly in female meiosis.
A. M. Meireles, K. H. Fisher, N. Colombie, J. G. Wakefield, and H. Ohkura (2009)
J. Cell Biol. 184, 777-784
   Abstract »    Full Text »    PDF »
Kinesin-5-dependent Poleward Flux and Spindle Length Control in Drosophila Embryo Mitosis.
I. Brust-Mascher, P. Sommi, D. K. Cheerambathur, and J. M. Scholey (2009)
Mol. Biol. Cell 20, 1749-1762
   Abstract »    Full Text »    PDF »
Mars, a Drosophila protein related to vertebrate HURP, is required for the attachment of centrosomes to the mitotic spindle during syncytial nuclear divisions.
G. Zhang, M. Breuer, A. Forster, D. Egger-Adam, and A. Wodarz (2009)
J. Cell Sci. 122, 535-545
   Abstract »    Full Text »    PDF »
Genome-wide analysis reveals a cell cycle-dependent mechanism controlling centromere propagation.
S. Erhardt, B. G. Mellone, C. M. Betts, W. Zhang, G. H. Karpen, and A. F. Straight (2008)
J. Cell Biol. 183, 805-818
   Abstract »    Full Text »    PDF »
Drosophila asterless and Vertebrate Cep152 Are Orthologs Essential for Centriole Duplication.
S. Blachon, J. Gopalakrishnan, Y. Omori, A. Polyanovsky, A. Church, D. Nicastro, J. Malicki, and T. Avidor-Reiss (2008)
Genetics 180, 2081-2094
   Abstract »    Full Text »    PDF »
{alpha}-Endosulfine is a conserved protein required for oocyte meiotic maturation in Drosophila.
J. R. Von Stetina, S. Tranguch, S. K. Dey, L. A. Lee, B. Cha, and D. Drummond-Barbosa (2008)
Development 135, 3697-3706
   Abstract »    Full Text »    PDF »
Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle.
G. Goshima, M. Mayer, N. Zhang, N. Stuurman, and R. D. Vale (2008)
J. Cell Biol. 181, 421-429
   Abstract »    Full Text »    PDF »
Dual roles of Incenp crucial to the assembly of the acentrosomal metaphase spindle in female meiosis.
N. Colombie, C. F. Cullen, A. L. Brittle, J. K. Jang, W. C. Earnshaw, M. Carmena, K. McKim, and H. Ohkura (2008)
Development 135, 3239-3246
   Abstract »    Full Text »    PDF »
mei-38 Is Required for Chromosome Segregation During Meiosis in Drosophila Females.
C. Wu, V. Singaram, and K. S. McKim (2008)
Genetics 180, 61-72
   Abstract »    Full Text »    PDF »
Mechanisms to suppress multipolar divisions in cancer cells with extra centrosomes.
M. Kwon, S. A. Godinho, N. S. Chandhok, N. J. Ganem, A. Azioune, M. Thery, and D. Pellman (2008)
Genes & Dev. 22, 2189-2203
   Abstract »    Full Text »    PDF »
A Multicomponent Assembly Pathway Contributes to the Formation of Acentrosomal Microtubule Arrays in Interphase Drosophila Cells.
G. C. Rogers, N. M. Rusan, M. Peifer, and S. L. Rogers (2008)
Mol. Biol. Cell 19, 3163-3178
   Abstract »    Full Text »    PDF »
Regulation of Microtubule Assembly and Organization in Mitosis by the AAA+ ATPase Pontin.
D. Ducat, S.-i. Kawaguchi, H. Liu, J. R. Yates III, and Y. Zheng (2008)
Mol. Biol. Cell 19, 3097-3110
   Abstract »    Full Text »    PDF »
GSK-3{beta} Regulates Proper Mitotic Spindle Formation in Cooperation with a Component of the {gamma}-Tubulin Ring Complex, GCP5.
N. Izumi, K. Fumoto, S. Izumi, and A. Kikuchi (2008)
J. Biol. Chem. 283, 12981-12991
   Abstract »    Full Text »    PDF »
Binding of ATP to UAP56 is necessary for mRNA export.
K. P. Kota, S. R. Wagner, E. Huerta, J. M. Underwood, and J. A. Nickerson (2008)
J. Cell Sci. 121, 1526-1537
   Abstract »    Full Text »    PDF »
Mob4 plays a role in spindle focusing in Drosophila S2 cells.
M. A. Trammell, N. M. Mahoney, D. A. Agard, and R. D. Vale (2008)
J. Cell Sci. 121, 1284-1292
   Abstract »    Full Text »    PDF »
RASSF7 Is a Member of a New Family of RAS Association Domain-containing Proteins and Is Required for Completing Mitosis.
V. Sherwood, R. Manbodh, C. Sheppard, and A. D. Chalmers (2008)
Mol. Biol. Cell 19, 1772-1782
   Abstract »    Full Text »    PDF »
Median Absolute Deviation to Improve Hit Selection for Genome-Scale RNAi Screens.
N. Chung, X. D. Zhang, A. Kreamer, L. Locco, P.-F. Kuan, S. Bartz, P. S. Linsley, M. Ferrer, and B. Strulovici (2008)
J Biomol Screen 13, 149-158
   Abstract »    PDF »
Getting in Sync with Dimeric Eg5: INITIATION AND REGULATION OF THE PROCESSIVE RUN.
T. C. Krzysiak, M. Grabe, and S. P. Gilbert (2008)
J. Biol. Chem. 283, 2078-2087
   Abstract »    Full Text »    PDF »
Genomic profiling and expression studies reveal both positive and negative activities for the Drosophila Myb MuvB/dREAM complex in proliferating cells.
D. Georlette, S. Ahn, D. M. MacAlpine, E. Cheung, P. W. Lewis, E. L. Beall, S. P. Bell, T. Speed, J. R. Manak, and M. R. Botchan (2007)
Genes & Dev. 21, 2880-2896
   Abstract »    Full Text »    PDF »
High throughput microscopy: from raw images to discoveries.
R. Wollman and N. Stuurman (2007)
J. Cell Sci. 120, 3715-3722
   Abstract »    Full Text »    PDF »
Tousled-like kinase in a microbial eukaryote regulates spindle assembly and S-phase progression by interacting with Aurora kinase and chromatin assembly factors.
Z. Li, S. Gourguechon, and C. C. Wang (2007)
J. Cell Sci. 120, 3883-3894
   Abstract »    Full Text »    PDF »
Revisiting the Role of the Mother Centriole in Centriole Biogenesis.
A. Rodrigues-Martins, M. Riparbelli, G. Callaini, D. M. Glover, and M. Bettencourt-Dias (2007)
Science 316, 1046-1050
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)