Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
The plant hormone abscisic acid (ABA) regulates many physiologicaland developmental processes in plants. The mechanism of ABAperception at the cell surface is not understood. Here, we reportthat a G proteincoupled receptor genetically and physicallyinteracts with the G protein subunit GPA1 to mediate all knownABA responses in Arabidopsis. Overexpressing this receptor resultsin an ABA-hypersensitive phenotype. This receptor binds ABAwith high affinity at physiological concentration with expectedkinetics and stereospecificity. The binding of ABA to the receptorleads to the dissociation of the receptor-GPA1 complex in yeast.Our results demonstrate that this G proteincoupled receptoris a plasma membrane ABA receptor.
1 National Institute of Biological Sciences, 7 Science Park Road, Zhongguancun Life Science Park, Beijing 102206, China. 2 Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, Hebei 050016, China. 3 State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
* To whom correspondence should be addressed. E-mail: maligeng{at}nibs.ac.cn
The editors suggest the following Related Resources on Science sites:
In Science Magazine
TECHNICAL COMMENTS
Christopher A. Johnston, Brenda R. Temple, Jin-Gui Chen, Yajun Gao, Etsuko N. Moriyama, Alan M. Jones, David P. Siderovski, and Francis S. Willard (9 November 2007) Science318 (5852), 914c.
[DOI: 10.1126/science.1143230] |Abstract »|Full Text »|PDF »
TECHNICAL COMMENTS
Xigang Liu, Yanling Yue, Wei Li, and Ligeng Ma (9 November 2007) Science318 (5852), 914d.
[DOI: 10.1126/science.1143320] |Abstract »|Full Text »|PDF »
PERSPECTIVES
Erwin Grill and Alexander Christmann (23 March 2007) Science315 (5819), 1676.
[DOI: 10.1126/science.1140761] |Summary »|Full Text »|PDF »
In Science Signaling
EDITORS' CHOICE
Pamela J. Hines (27 March 2007) Sci. STKE2007 (379), tw106.
[DOI: 10.1126/stke.3792007tw106] |Abstract »
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
RACK1 is a negative regulator of ABA responses in Arabidopsis.
J. Guo, J. Wang, L. Xi, W.-D. Huang, J. Liang, and J.-G. Chen (2009)
J. Exp. Bot.
|Abstract »|Full Text »|PDF »
Abscisic Acid Activates the Murine Microglial Cell Line N9 through the Second Messenger Cyclic ADP-ribose.
N. Bodrato, L. Franco, C. Fresia, L. Guida, C. Usai, A. Salis, I. Moreschi, C. Ferraris, C. Verderio, G. Basile, et al. (2009)
J. Biol. Chem.
284, 14777-14787
|Abstract »|Full Text »|PDF »
Regulators of PP2C Phosphatase Activity Function as Abscisic Acid Sensors.
Y. Ma, I. Szostkiewicz, A. Korte, D. Moes, Y. Yang, A. Christmann, and E. Grill (2009)
Science
324, 1064-1068
|Abstract »|Full Text »|PDF »
Abscisic Acid Inhibits Type 2C Protein Phosphatases via the PYR/PYL Family of START Proteins.
S.-Y. Park, P. Fung, N. Nishimura, D. R. Jensen, H. Fujii, Y. Zhao, S. Lumba, J. Santiago, A. Rodrigues, T.-f. F. Chow, et al. (2009)
Science
324, 1068-1071
|Abstract »|Full Text »|PDF »
A Novel ABA Insensitive Mutant of Lotus japonicus with a Wilty Phenotype Displays Unaltered Nodulation Regulation.
A Signaling Pathway Linking Nitric Oxide Production to Heterotrimeric G Protein and Hydrogen Peroxide Regulates Extracellular Calmodulin Induction of Stomatal Closure in Arabidopsis.
J.-H. Li, Y.-Q. Liu, P. Lu, H.-F. Lin, Y. Bai, X.-C. Wang, and Y.-L. Chen (2009)
Plant Physiology
150, 114-124
|Abstract »|Full Text »|PDF »
The guard cell as a single-cell model towards understanding drought tolerance and abscisic acid action.
C. Sirichandra, A. Wasilewska, F. Vlad, C. Valon, and J. Leung (2009)
J. Exp. Bot.
60, 1439-1463
|Abstract »|Full Text »|PDF »
The primary signaling outputs of brassinosteroids are regulated by abscisic acid signaling.
Interactions between the S-Domain Receptor Kinases and AtPUB-ARM E3 Ubiquitin Ligases Suggest a Conserved Signaling Pathway in Arabidopsis.
M. A. Samuel, Y. Mudgil, J. N. Salt, F. Delmas, S. Ramachandran, A. Chilelli, and D. R. Goring (2008)
Plant Physiology
147, 2084-2095
|Abstract »|Full Text »|PDF »
Loss-of-Function Mutations in the Arabidopsis Heterotrimeric G-protein {alpha} Subunit Enhance the Developmental Defects of Brassinosteroid Signaling and Biosynthesis Mutants.
Y. Gao, S. Wang, T. Asami, and J.-G. Chen (2008)
Plant Cell Physiol.
49, 1013-1024
|Abstract »|Full Text »|PDF »
G{gamma}1 + G{gamma}2 != G{beta}: Heterotrimeric G Protein G{gamma}-Deficient Mutants Do Not Recapitulate All Phenotypes of G{beta}-Deficient Mutants.
Y. Trusov, W. Zhang, S. M. Assmann, and J. R. Botella (2008)
Plant Physiology
147, 636-649
|Abstract »|Full Text »|PDF »
Characterization of the Arabidopsis Heterotrimeric G Protein.
S. Wang, S. M. Assmann, and N. V. Fedoroff (2008)
J. Biol. Chem.
283, 13913-13922
|Abstract »|Full Text »|PDF »
Back from the Dormant Stage: Second Messenger Cyclic ADP-Ribose Essential for Toxoplasma gondii Pathogenicity.
Activated Expression of an Arabidopsis HD-START Protein Confers Drought Tolerance with Improved Root System and Reduced Stomatal Density.
H. Yu, X. Chen, Y.-Y. Hong, Y. Wang, P. Xu, S.-D. Ke, H.-Y. Liu, J.-K. Zhu, D. J. Oliver, and C.-B. Xiang (2008)
PLANT CELL
20, 1134-1151
|Abstract »|Full Text »|PDF »
An Update on Abscisic Acid Signaling in Plants and More ....
A. Wasilewska, F. Vlad, C. Sirichandra, Y. Redko, F. Jammes, C. Valon, N. F. d. Frey, and J. Leung (2008)
Mol Plant
1, 198-217
|Abstract »|Full Text »|PDF »
Characterization of Gibberellin Receptor Mutants of Barley (Hordeum vulgare L.).
P. M. Chandler, C. A. Harding, A. R. Ashton, M. D. Mulcair, N. E. Dixon, and L. N. Mander (2008)
Mol Plant
1, 285-294
|Abstract »|Full Text »|PDF »
Hydrogen peroxide generated by copper amine oxidase is involved in abscisic acid-induced stomatal closure in Vicia faba.
Comment on "A G Protein Coupled Receptor Is a Plasma Membrane Receptor for the Plant Hormone Abscisic Acid".
C. A. Johnston, B. R. Temple, J.-G. Chen, Y. Gao, E. N. Moriyama, A. M. Jones, D. P. Siderovski, and F. S. Willard (2007)
Science
318, 914c
|Abstract »|Full Text »|PDF »
Response to Comment on "A G Protein Coupled Receptor Is a Plasma Membrane Receptor for the Plant Hormone Abscisic Acid".
GTPase acceleration as the rate-limiting step in Arabidopsis G protein-coupled sugar signaling.
C. A. Johnston, J. P. Taylor, Y. Gao, A. J. Kimple, J. C. Grigston, J.-G. Chen, D. P. Siderovski, A. M. Jones, and F. S. Willard (2007)
PNAS
104, 17317-17322
|Abstract »|Full Text »|PDF »
Two Calcium-Dependent Protein Kinases, CPK4 and CPK11, Regulate Abscisic Acid Signal Transduction in Arabidopsis.
S.-Y. Zhu, X.-C. Yu, X.-J. Wang, R. Zhao, Y. Li, R.-C. Fan, Y. Shang, S.-Y. Du, X.-F. Wang, F.-Q. Wu, et al. (2007)
PLANT CELL
19, 3019-3036
|Abstract »|Full Text »|PDF »
N-Acylethanolamine Metabolism Interacts with Abscisic Acid Signaling in Arabidopsis thaliana Seedlings.
N. D. Teaster, C. M. Motes, Y. Tang, W. C. Wiant, M. Q. Cotter, Y.-S. Wang, A. Kilaru, B. J. Venables, K. H. Hasenstein, G. Gonzalez, et al. (2007)
PLANT CELL
19, 2454-2469
|Abstract »|Full Text »|PDF »