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Science 2 March 2007:
Vol. 315. no. 5816, pp. 1270 - 1274
DOI: 10.1126/science.1138527
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Reports
Reconstitution of DNA Segregation Driven by Assembly of a Prokaryotic Actin Homolog
Ethan C. Garner,1,2,3
Christopher S. Campbell,1,2,3
Douglas B. Weibel,3,4
R. Dyche Mullins1,2,3*
Multiple unrelated polymer systems have evolved to partition DNA molecules between daughter cells at division. To better understand polymer-driven DNA segregation, we reconstituted the three-component segregation system of the R1 plasmid from purified components. We found that the ParR/parC complex can construct a simple bipolar spindle by binding the ends of ParM filaments, inhibiting dynamic instability, and acting as a ratchet permitting incorporation of new monomers and riding on the elongating filament ends. Under steady-state conditions, the dynamic instability of unattached ParM filaments provides the energy required to drive DNA segregation.
1 Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA.
2 UCSF/UCB Nanomedicine Development Center, San Francisco, CA 94158, USA.
3 Physiology Course, Marine Biological Laboratory, Woods Hole, MA02543, USA.
4 Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA.
* To whom correspondence should be addressed. E-mail: dyche{at}mullinslab.ucsf.edu
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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
- Streptococcus pyogenes pSM19035 requires dynamic assembly of ATP-bound ParA and ParB on parS DNA during plasmid segregation.
- F. Pratto, A. Cicek, W. A. Weihofen, R. Lurz, W. Saenger, and J. C. Alonso (2008)
Nucleic Acids Res.
36, 3676-3689
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- In Vitro Assembly Studies of FtsZ/Tubulin-like Proteins (TubZ) from Bacillus Plasmids: EVIDENCE FOR A CAPPING MECHANISM.
- Y. Chen and H. P. Erickson (2008)
J. Biol. Chem.
283, 8102-8109
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- Bacterial Growth and Cell Division: a Mycobacterial Perspective.
- E. C. Hett and E. J. Rubin (2008)
Microbiol. Mol. Biol. Rev.
72, 126-156
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- Centromere anatomy in the multidrug-resistant pathogen Enterococcus faecium.
- A. Derome, C. Hoischen, M. Bussiek, R. Grady, M. Adamczyk, B. Keedzierska, S. Diekmann, D. Barilla, and F. Hayes (2008)
PNAS
105, 2151-2156
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- Escherichia coli low-copy-number plasmid R1 centromere parC forms a U-shaped complex with its binding protein ParR.
- C. Hoischen, M. Bussiek, J. Langowski, and S. Diekmann (2008)
Nucleic Acids Res.
36, 607-615
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- Plasmid segregation: spatial awareness at the molecular level.
- J. Moller-Jensen and K. Gerdes (2007)
J. Cell Biol.
179, 813-815
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- In vivo visualization of type II plasmid segregation: bacterial actin filaments pushing plasmids.
- C. S. Campbell and R. D. Mullins (2007)
J. Cell Biol.
179, 1059-1066
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- Distribution of Centromere-Like parS Sites in Bacteria: Insights from Comparative Genomics.
- J. Livny, Y. Yamaichi, and M. K. Waldor (2007)
J. Bacteriol.
189, 8693-8703
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- Changes in Nucleoid Morphology and Origin Localization upon Inhibition or Alteration of the Actin Homolog, MreB, of Vibrio cholerae.
- P. Srivastava, G. Demarre, T. S. Karpova, J. McNally, and D. K. Chattoraj (2007)
J. Bacteriol.
189, 7450-7463
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- Centromere Pairing by a Plasmid-encoded Type I ParB Protein.
- S. Ringgaard, J. Lowe, and K. Gerdes (2007)
J. Biol. Chem.
282, 28216-28225
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- Treadmilling of a prokaryotic tubulin-like protein, TubZ, required for plasmid stability in Bacillus thuringiensis.
- R. A. Larsen, C. Cusumano, A. Fujioka, G. Lim-Fong, P. Patterson, and J. Pogliano (2007)
Genes & Dev.
21, 1340-1352
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