Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 16 February 2007:
Vol. 315. no. 5814, pp. 988 - 992
DOI: 10.1126/science.1136606
Prev | Table of Contents | Next

Reports

Multipotent Drosophila Intestinal Stem Cells Specify Daughter Cell Fates by Differential Notch Signaling

Benjamin Ohlstein and Allan Spradling*

The adult Drosophila midgut contains multipotent intestinal stem cells (ISCs) scattered along its basement membrane that have been shown by lineage analysis to generate both enterocytes and enteroendocrine cells. ISCs containing high levels of cytoplasmic Delta-rich vesicles activate the canonical Notch pathway and down-regulate Delta within their daughters, a process that programs these daughters to become enterocytes. ISCs that express little vesiculate Delta, or are genetically impaired in Notch signaling, specify their daughters to become enteroendocrine cells. Thus, ISCs control daughter cell fate by modulating Notch signaling over time. Our studies suggest that ISCs actively coordinate cell production with local tissue requirements by this mechanism.

Howard Hughes Medical Institute, Department of Embryology, Carnegie Institution of Washington, 3520 San Martin Drive, Baltimore, MD 21218, USA.

* To whom correspondence should be addressed. E-mail: spradling{at}ciwemb.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Bacterial-modulated host immunity and stem cell activation for gut homeostasis.
W.-J. Lee (2009)
Genes & Dev. 23, 2260-2265
   Abstract »    Full Text »    PDF »
Paracrine Unpaired Signaling through the JAK/STAT Pathway Controls Self-renewal and Lineage Differentiation of Drosophila Intestinal Stem Cells.
G. Lin, N. Xu, and R. Xi (2009)
J Mol Cell Biol
   Abstract »    Full Text »    PDF »
Basal cells as stem cells of the mouse trachea and human airway epithelium.
J. R. Rock, M. W. Onaitis, E. L. Rawlins, Y. Lu, C. P. Clark, Y. Xue, S. H. Randell, and B. L. M. Hogan (2009)
PNAS 106, 12771-12775
   Abstract »    Full Text »    PDF »
Adenomatous polyposis coli regulates Drosophila intestinal stem cell proliferation.
W.-C. Lee, K. Beebe, L. Sudmeier, and C. A. Micchelli (2009)
Development 136, 2255-2264
   Abstract »    Full Text »    PDF »
EGFR signaling regulates the proliferation of Drosophila adult midgut progenitors.
H. Jiang and B. A. Edgar (2009)
Development 136, 483-493
   Abstract »    Full Text »    PDF »
Drosophila Stem Cells Share a Common Requirement for the Histone H2B Ubiquitin Protease Scrawny.
M. Buszczak, S. Paterno, and A. C. Spradling (2009)
Science 323, 248-251
   Abstract »    Full Text »    PDF »
Stem Cells, Their Niches and the Systemic Environment: An Aging Network.
D. Drummond-Barbosa (2008)
Genetics 180, 1787-1797
   Abstract »    Full Text »    PDF »
E-cadherin prolongs the moment for interaction between intestinal stem cell and its progenitor cell to ensure Notch signaling in adult Drosophila midgut.
K. Maeda, M. Takemura, M. Umemori, and T. Adachi-Yamada (2008)
Genes Cells 13, 1219-1227
   Abstract »    Full Text »    PDF »
Stem Cells and Their Niches: Integrated Units That Maintain Drosophila Tissues.
A.C. Spradling, T. Nystul, D. Lighthouse, L. Morris, D. Fox, R. Cox, T. Tootle, R. Frederick, and A. Skora (2008)
Cold Spring Harb Symp Quant Biol
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)