Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 9 February 2007:
Vol. 315. no. 5813, pp. 843 - 848
DOI: 10.1126/science.1135710
Prev | Table of Contents | Next

Reports

Structure of the Prefusion Form of the Vesicular Stomatitis Virus Glycoprotein G

Stéphane Roche, Félix A. Rey,* Yves Gaudin,{dagger} Stéphane Bressanelli

Glycoprotein G of the vesicular stomatitis virus triggers membrane fusion via a low pH–induced structural rearrangement. Despite the equilibrium between the pre- and postfusion states, the structure of the prefusion form, determined to 3.0 angstrom resolution, shows that the fusogenic transition entails an extensive structural reorganization of G. Comparison with the structure of the postfusion form suggests a pathway for the conformational change. In the prefusion form, G has the shape of a tripod with the fusion loops exposed, which point toward the viral membrane, and with the antigenic sites located at the distal end of the molecule. A large number of G glycoproteins, perhaps organized as in the crystals, act cooperatively to induce membrane merging.

CNRS, Unité Mixte de Recherche (UMR) 2472, Institut National de la Recherche Agronomique (INRA), UMR 1157, Institut Fédératif de Recherche 115, Laboratoire de Virologie Moléculaire et Structurale, 91198, Gif sur Yvette, France.

* Present address: Département de Virologie, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris cedex 15, France.

{dagger} To whom correspondence should be addressed. E-mail: gaudin{at}vms.cnrs-gif.fr

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Role of Conserved Histidine Residues in the Low-pH Dependence of the Semliki Forest Virus Fusion Protein.
Z.-l. Qin, Y. Zheng, and M. Kielian (2009)
J. Virol. 83, 4670-4677
   Abstract »    Full Text »    PDF »
Glycoprotein L sets the neutralization profile of murid herpesvirus 4.
L. Gillet, M. Alenquer, D. L. Glauser, S. Colaco, J. S. May, and P. G. Stevenson (2009)
J. Gen. Virol. 90, 1202-1214
   Abstract »    Full Text »    PDF »
Structure of a trimeric variant of the Epstein-Barr virus glycoprotein B.
M. Backovic, R. Longnecker, and T. S. Jardetzky (2009)
PNAS 106, 2880-2885
   Abstract »    Full Text »    PDF »
Analysis of Epstein-Barr Virus Glycoprotein B Functional Domains via Linker Insertion Mutagenesis.
J. J. Reimer, M. Backovic, C. G. Deshpande, T. Jardetzky, and R. Longnecker (2009)
J. Virol. 83, 734-747
   Abstract »    Full Text »    PDF »
Native 3D intermediates of membrane fusion in herpes simplex virus 1 entry.
U. E. Maurer, B. Sodeik, and K. Grunewald (2008)
PNAS 105, 10559-10564
   Abstract »    Full Text »    PDF »
Unique Small Molecule Entry Inhibitors of Hemorrhagic Fever Arenaviruses.
A. M. Lee, J. M. Rojek, C. F. Spiropoulou, A. T. Gundersen, W. Jin, A. Shaginian, J. York, J. H. Nunberg, D. L. Boger, M. B. A. Oldstone, et al. (2008)
J. Biol. Chem. 283, 18734-18742
   Abstract »    Full Text »    PDF »
Glycoprotein B switches conformation during murid herpesvirus 4 entry.
L. Gillet, S. Colaco, and P. G. Stevenson (2008)
J. Gen. Virol. 89, 1352-1363
   Abstract »    Full Text »    PDF »
A fusion-intermediate state of HIV-1 gp41 targeted by broadly neutralizing antibodies.
G. Frey, H. Peng, S. Rits-Volloch, M. Morelli, Y. Cheng, and B. Chen (2008)
PNAS 105, 3739-3744
   Abstract »    Full Text »    PDF »
Molecular Architecture of the Bipartite Fusion Loops of Vesicular Stomatitis Virus Glycoprotein G, a Class III Viral Fusion Protein.
X. Sun, S. Belouzard, and G. R. Whittaker (2008)
J. Biol. Chem. 283, 6418-6427
   Abstract »    Full Text »    PDF »
Positively selected sites on the surface glycoprotein (G) of infectious hematopoietic necrosis virus.
S. E. LaPatra, C. Evilia, and V. Winston (2008)
J. Gen. Virol. 89, 703-708
   Abstract »    Full Text »    PDF »
Characterization of Fusion Determinants Points to the Involvement of Three Discrete Regions of Both E1 and E2 Glycoproteins in the Membrane Fusion Process of Hepatitis C Virus.
D. Lavillette, E.-I. Pecheur, P. Donot, J. Fresquet, J. Molle, R. Corbau, M. Dreux, F. Penin, and F.-L. Cosset (2007)
J. Virol. 81, 8752-8765
   Abstract »    Full Text »    PDF »
Random linker-insertion mutagenesis to identify functional domains of herpes simplex virus type 1 glycoprotein B.
E. Lin and P. G. Spear (2007)
PNAS 104, 13140-13145
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)