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Science 9 February 2007:
Vol. 315. no. 5813, p. 766
DOI: 10.1126/science.1135047
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Technical Comments

Comment on "Obestatin, a Peptide Encoded by the Ghrelin Gene, Opposes Ghrelin's Effects on Food Intake"

N. Chartrel,1* R. Alvear-Perez,2* J. Leprince,1 X. Iturrioz,2 A. Reaux-Le Goazigo,2 V. Audinot,3 P. Chomarat,3 F. Coge,3 O. Nosjean,3 M. Rodriguez,3 J. P. Galizzi,3 J. A. Boutin,3{dagger} H. Vaudry,1{dagger} C. Llorens-Cortes2{dagger}

Zhang et al. (Research Articles, 11 November 2005, p. 996) reported that obestatin, a peptide derived from the ghrelin precursor, activated the orphan G protein–coupled receptor GPR39. However, we found that I125-obestatin does not bind GPR39 and observed no effects of obestatin on GPR39-transfected cells in various functional assays (cyclic adenosine monophosphate production, calcium mobilization, and GPR39 internalization). Our results indicate that obestatin is not the cognate ligand for GPR39.

1 Institut National de la Santé et de la Recherche Médicale (INSERM), U413, Laboratory of Cellular and Molecular Neuroendocrinology, and European Institute for Peptide Research (IFRMP 23), University of Rouen, 76821 Mont-Saint-Aignan, France.
2 INSERM, U691, and Collège de France, 75005 Paris, France.
3 Institut de Recherches Servier (IdRS), Centre de Recherches de Croissy, 125 Chemin de la Ronde, 78290 Croissy-sur-Seine, France.

* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: c.llorens-cortes{at}college-de-france.fr (C.L.C.); hubert.vaudry{at}univ-rouen.fr (H.V.); jean.boutin{at}fr.netgrs.com (J.A.B.)

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Two alternatively spliced GPR39 transcripts in seabream: molecular cloning, genomic organization, and regulation of gene expression by metabolic signals.
Y. Zhang, Y. Liu, X. Huang, X. Liu, B. Jiao, Z. Meng, P. Zhu, S. Li, H. Lin, and C. H K Cheng (2008)
J. Endocrinol. 199, 457-470
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