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Science 2 February 2007:
Vol. 315. no. 5812, pp. 646 - 649
DOI: 10.1126/science.1134641
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Reports

The DEAD-Box RNA Helicase Dbp5 Functions in Translation Termination

Thomas Gross,1 Anja Siepmann,1 Dorotheé Sturm,1 Merle Windgassen,1* John J. Scarcelli,2 Matthias Seedorf,3 Charles N. Cole,2 Heike Krebber1{dagger}

In eukaryotes, termination of messenger RNA (mRNA) translation is mediated by the release factors eRF1 and eRF3. Using Saccharomyces cerevisiae as a model organism, we have identified a member of the DEAD-box protein (DBP) family, the DEAD-box RNA helicase and mRNA export factor Dbp5, as a player in translation termination. Dbp5 interacts genetically with both release factors and the polyadenlyate-binding protein Pab1. A physical interaction was specifically detected with eRF1. Moreover, we show that the helicase activity of Dbp5 is required for efficient stop-codon recognition, and intact Dbp5 is essential for recruitment of eRF3 into termination complexes. Therefore, Dbp5 controls the eRF3-eRF1 interaction and thus eRF3-mediated downstream events.

1 Institut für Molekularbiologie und Tumorforschung der Philipps-Universität Marburg, Emil-Mannkopff-Strasse 2, 35037 Marburg, Germany.
2 Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA.
3 Zentrum für Molekulare Biologie der Universität in Heidelberg, Im Neuenheimer Feld 282, 69129 Heidelberg, Germany.

* Present address: Medical Biotechnology Center (MBC) of the University of Southern Denmark, Winsløwparken 25, 5000 Odense C, Denmark.

{dagger} To whom correspondence should be addressed. E-mail: krebber{at}imt.uni-marburg.de

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Site-Specific Release of Nascent Chains from Ribosomes at a Sense Codon.
V. A. Doronina, C. Wu, P. de Felipe, M. S. Sachs, M. D. Ryan, and J. D. Brown (2008)
Mol. Cell. Biol. 28, 4227-4239
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Effect of NS3 and NS5B proteins on classical swine fever virus internal ribosome entry site-mediated translation and its host cellular translation.
M. Xiao, Y. Bai, H. Xu, X. Geng, J. Chen, Y. Wang, J. Chen, and B. Li (2008)
J. Gen. Virol. 89, 994-999
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Definition of global and transcript-specific mRNA export pathways in metazoans.
N. G. Farny, J. A. Hurt, and P. A. Silver (2008)
Genes & Dev. 22, 66-78
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Science. ISSN 0036-8075 (print), 1095-9203 (online)