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Science 12 January 2007:
Vol. 315. no. 5809, pp. 253 - 255
DOI: 10.1126/science.1136264
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Reports

Picobiliphytes: A Marine Picoplanktonic Algal Group with Unknown Affinities to Other Eukaryotes

Fabrice Not,1*{ddagger}§ Klaus Valentin,2{ddagger} Khadidja Romari,1{dagger} Connie Lovejoy,3 Ramon Massana,4 Kerstin Töbe,2 Daniel Vaulot,1 Linda K. Medlin2§

Environmental sequencing has revealed unimagined diversity among eukaryotic picoplankton. A distinct picoplanktonic algal group, initially detected from 18S ribosomal DNA (rDNA) sequences, was hybridized with rRNA {lambda}-targeted (rRNA-targeted) probes, detected by tyramide signal amplification–fluorescent in situ hybridization, and showed an organelle-like body with orange fluorescence indicative of phycobilins. Using this fluorescence signal, cells were sorted by flow cytometry and probed. Hybridized cells contained a 4',6'-diamidino-2-phenylindole–stained organelle resembling a plastid with a nucleomorph. This suggests that they may be secondary endosymbiotic algae. Pending the isolation of living cells and their formal description, these algae have been termed picobiliphytes.

1 Station Biologique de Roscoff, UMR 7144 CNRS and Université Pierre et Marie Curie, Boîte Postale 74, 29682 Roscoff Cedex, France.
2 Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, D.27570 Bremerhaven, Germany.
3 Québec Ocean, Département de Biologie, Université Laval, Quebec, QC Canada G1K 7P4.
4 Institut de Ciències del Mar, Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Spain.

* Present address: Institut de Ciències del Mar, Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Spain.

{ddagger} These authors contributed equally to this work.

{dagger} Present address: Albany Molecular Research, BRC 18804 North Creek Parkway, Bothell, WA 98011–8012, USA.

§ To whom correspondence should be addressed. E-mail: lkmedlin{at}awi-bremerhaven.de (L.M.); not{at}icm.csic.es (F.N.)

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A Cryptic Algal Group Unveiled: A Plastid Biosynthesis Pathway in the Oyster Parasite Perkinsus marinus.
M. Matsuzaki, H. Kuroiwa, T. Kuroiwa, K. Kita, and H. Nozaki (2008)
Mol. Biol. Evol. 25, 1167-1179
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Molecular systematics of the Pleurochrysidaceae, a family of coastal coccolithophores (Haptophyta).
A. G. Saez, A. Zaldivar-Riveron, and L. K. Medlin (2008)
J. Plankton Res. 30, 559-566
   Abstract »    Full Text »    PDF »
Phylogenomic Analysis Supports the Monophyly of Cryptophytes and Haptophytes and the Association of Rhizaria with Chromalveolates.
J. D. Hackett, H. S. Yoon, S. Li, A. Reyes-Prieto, S. E. Rummele, and D. Bhattacharya (2007)
Mol. Biol. Evol. 24, 1702-1713
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Science. ISSN 0036-8075 (print), 1095-9203 (online)