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Differential Transmission of Actin Motion Within Focal Adhesions
Ke Hu,*Lin Ji,*Kathryn T. Applegate,Gaudenz Danuser,Clare M. Waterman-Storer
Cell migration requires the transmission of motion generatedin the actin cytoskeleton to the extracellular environment througha complex assembly of proteins in focal adhesions. We developedcorrelational fluorescent speckle microscopy to measure thecoupling of focal-adhesion proteins to actin filaments. Differentclasses of focal-adhesion structural and regulatory moleculesexhibited varying degrees of correlated motions with actin filaments,indicating hierarchical transmission of actin motion throughfocal adhesions. Interactions between vinculin, talin, and actinfilaments appear to constitute a slippage interface betweenthe cytoskeleton and integrins, generating a molecular clutchthat is regulated during the morphodynamic transitions of cellmigration.
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
* These authors contributed equally to this work.
To whom correspondence should be addressed. E-mail: waterman{at}scripps.edu (C.M.W.-S.); gdanuser{at}scripps.edu (G.D.)
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To whom correspondence should be addressed. E-mail: 
