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Science 22 December 2006:
Vol. 314. no. 5807, p. 1893
DOI: 10.1126/science.1135268
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Brevia

Untemplated Oligoadenylation Promotes Degradation of RISC-Cleaved Transcripts

Fadia Ibrahim,* Jennifer Rohr,* Won-Joong Jeong, Jennifer Hesson, Heriberto Cerutti{dagger}

Double-stranded RNA, processed to small interfering RNAs (siRNAs) by Dicer and incorporated into the RNA-induced silencing complex (RISC), triggers gene silencing by a variety of pathways in eukaryotes. RNA interference involving the degradation of homologous transcripts is the best-characterized mechanism. However, the fate of the RNA fragments resulting from siRNA-directed cleavage is poorly understood. We have identified a gene (MUT68) in the unicellular green alga Chlamydomonas reinhardtii that is required for the efficient decay of siRNA-targeted transcripts. MUT68 encodes a noncanonical polyadenylate polymerase that adds untemplated adenines to the 5' RNA fragments after siRNA-mediated cleavage and appears to stimulate their exosome-dependent degradation.

Biological Sciences and Plant Science Initiative, University of Nebraska, Lincoln, NE 68588, USA.

* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: hcerutti{at}unl.edu

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Degradation of histone mRNA requires oligouridylation followed by decapping and simultaneous degradation of the mRNA both 5' to 3' and 3' to 5'.
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Science. ISSN 0036-8075 (print), 1095-9203 (online)