Related Content
Search Google Scholar for:
More Information
Related Jobs from ScienceCareers
|
|
Science 8 December 2006: Vol. 314. no. 5805, pp. 1565 - 1568 DOI: 10.1126/science.1131969
|
|
Research Articles
Engineering Yeast Transcription Machinery for Improved Ethanol Tolerance and Production
Hal Alper,1,3
Joel Moxley,1
Elke Nevoigt,1,2
Gerald R. Fink,3
Gregory Stephanopoulos1*
Global transcription machinery engineering (gTME) is an approach for reprogramming gene transcription to elicit cellular phenotypes important for technological applications. Here we show the application of gTME to Saccharomyces cerevisiae for improved glucose/ethanol tolerance, a key trait for many biofuels programs. Mutagenesis of the transcription factor Spt15p and selection led to dominant mutations that conferred increased tolerance and more efficient glucose conversion to ethanol. The desired phenotype results from the combined effect of three separate mutations in the SPT15 gene [serine substituted for phenylalanine (Phe 177Ser) and, similarly, Tyr 195His, and Lys 218Arg]. Thus, gTME can provide a route to complex phenotypes that are not readily accessible by traditional methods.
1 Department of Chemical Engineering, Massachusetts Institute of Technology, Room 56-469, Cambridge, MA 02139, USA.
2 Department of Microbiology and Genetics, Berlin University of Technology, Seestrasse 13, D-13353 Berlin, Germany.
3 Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA.
* To whom correspondence should be addressed. E-mail: gregstep{at}mit.edu
Read the Full Text
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
- Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides.
- D. G. Gibson (2009)
Nucleic Acids Res.
37, 6984-6990
| Abstract »
| Full Text »
| PDF »
- Impaired Uptake and/or Utilization of Leucine by Saccharomyces cerevisiae Is Suppressed by the SPT15-300 Allele of the TATA-Binding Protein Gene.
- R. J. S. Baerends, J.-L. Qiu, S. Rasmussen, H. B. Nielsen, and A. Brandt (2009)
Appl. Envir. Microbiol.
75, 6055-6061
| Abstract »
| Full Text »
| PDF »
- Genome-Wide Identification of Saccharomyces cerevisiae Genes Required for Maximal Tolerance to Ethanol.
- M. C. Teixeira, L. R. Raposo, N. P. Mira, A. B. Lourenco, and I. Sa-Correia (2009)
Appl. Envir. Microbiol.
75, 5761-5772
| Abstract »
| Full Text »
| PDF »
- Systems-Level Engineering of Nonfermentative Metabolism in Yeast.
- C. J. Kennedy, P. M. Boyle, Z. Waks, and P. A. Silver (2009)
Genetics
183, 385-397
| Abstract »
| Full Text »
| PDF »
- Harnessing nature's toolbox: regulatory elements for synthetic biology.
- P. M. Boyle and P. A. Silver (2009)
J R Soc Interface
6, S535-S546
| Abstract »
| Full Text »
| PDF »
- Engineering of an Escherichia coli Strain for the Production of 3-Methyl-1-Butanol.
- M. R. Connor and J. C. Liao (2008)
Appl. Envir. Microbiol.
74, 5769-5775
| Abstract »
| Full Text »
| PDF »
- Progress in Metabolic Engineering of Saccharomyces cerevisiae.
- E. Nevoigt (2008)
Microbiol. Mol. Biol. Rev.
72, 379-412
| Abstract »
| Full Text »
| PDF »
- Phenotypic engineering by reprogramming gene transcription using novel artificial transcription factors in Escherichia coli.
- J. Y. Lee, B. H. Sung, B. J. Yu, J. H. Lee, S. H. Lee, M. S. Kim, M. D. Koob, and S. C. Kim (2008)
Nucleic Acids Res.
36, e102
| Abstract »
| Full Text »
| PDF »
- Assessing the potential of mutational strategies to elicit new phenotypes in industrial strains.
- D. Klein-Marcuschamer and G. Stephanopoulos (2008)
PNAS
105, 2319-2324
| Abstract »
| Full Text »
| PDF »
- Dynamics of Genomic-Library Enrichment and Identification of Solvent Tolerance Genes for Clostridium acetobutylicum.
- J. R. Borden and E. T. Papoutsakis (2007)
Appl. Envir. Microbiol.
73, 3061-3068
| Abstract »
| Full Text »
| PDF »
- Challenges in Engineering Microbes for Biofuels Production.
- G. Stephanopoulos (2007)
Science
315, 801-804
| Abstract »
| Full Text »
| PDF »
|
|