Related Content
Search Google Scholar for:
More Information
Related Jobs from ScienceCareers
|
Originally published in Science Express on 23 November 2006
Science 1 December 2006: Vol. 314. no. 5804, pp. 1450 - 1454
DOI: 10.1126/science.1133281
|
|
Reports
Structural Basis for Ribosome Recruitment and Manipulation by a Viral IRES RNA
Jennifer S. Pfingsten,
David A. Costantino,
Jeffrey S. Kieft*
Canonical cap-dependent translation initiation requires a large number of protein factors that act in a stepwise assembly process. In contrast, internal ribosomal entry sites (IRESs) are cis-acting RNAs that in some cases completely supplant these factors by recruiting and activating the ribosome using a single structured RNA. Here we present the crystal structures of the ribosome-binding domain from a Dicistroviridae intergenic region IRES at 3.1 angstrom resolution, providing a view of the prefolded architecture of an all-RNA translation initiation apparatus. Docking of the structure into cryoelectron microscopy reconstructions of an IRES-ribosome complex suggests a model for ribosome manipulation by a dynamic IRES RNA.
Department of Biochemistry and Molecular Genetics, University of Colorado at Denver and Health Sciences Center, Mail Stop 8101, Post Office Box 6511, Aurora, CO 80045, USA.
* To whom correspondence should be addressed. E-mail: Jeffrey.Kieft{at}uchsc.edu
Read the Full Text
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
- Translation initiation factors are not required for Dicistroviridae IRES function in vivo.
- N. Deniz, E. M. Lenarcic, D. M. Landry, and S. R. Thompson (2009)
RNA
15, 932-946
| Abstract »
| Full Text »
| PDF »
- Analysis and classification of RNA tertiary structures.
- M. Abraham, O. Dror, R. Nussinov, and H. J. Wolfson (2008)
RNA
14, 2274-2289
| Abstract »
| Full Text »
| PDF »
- RNA structure-based ribosome recruitment: Lessons from the Dicistroviridae intergenic region IRESes.
- J. S. Pfingsten and J. S. Kieft (2008)
RNA
14, 1255-1263
| Abstract »
| Full Text »
| PDF »
- New insights into internal ribosome entry site elements relevant for viral gene expression.
- E. Martinez-Salas, A. Pacheco, P. Serrano, and N. Fernandez (2008)
J. Gen. Virol.
89, 611-626
| Abstract »
| Full Text »
| PDF »
- Roles of the negatively charged N-terminal extension of Saccharomyces cerevisiae ribosomal protein S5 revealed by characterization of a yeast strain containing human ribosomal protein S5.
- O. Galkin, A. A. Bentley, S. Gupta, B.-A. Compton, B. Mazumder, T. G. Kinzy, W. C. Merrick, M. Hatzoglou, T. V. Pestova, C. U.T. Hellen, et al. (2007)
RNA
13, 2116-2128
| Abstract »
| Full Text »
| PDF »
- In vivo footprint of a picornavirus internal ribosome entry site reveals differences in accessibility to specific RNA structural elements.
- O. Fernandez-Miragall and E. Martinez-Salas (2007)
J. Gen. Virol.
88, 3053-3062
| Abstract »
| Full Text »
| PDF »
- A Baculovirus-Expressed Dicistrovirus That Is Infectious to Aphids.
- N. Pal, S. Boyapalle, R. Beckett, W. A. Miller, and B. C. Bonning (2007)
J. Virol.
81, 9339-9345
| Abstract »
| Full Text »
| PDF »
- Characterization of a cyanobacterial RNase P ribozyme recognition motif in the IRES of foot-and-mouth disease virus reveals a unique structural element.
- P. Serrano, J. Gomez, and E. Martinez-Salas (2007)
RNA
13, 849-859
| Abstract »
| Full Text »
| PDF »
- Eukaryotic ribosomal protein RPS25 interacts with the conserved loop region in a dicistroviral intergenic internal ribosome entry site.
- T. Nishiyama, H. Yamamoto, T. Uchiumi, and N. Nakashima (2007)
Nucleic Acids Res.
35, 1514-1521
| Abstract »
| Full Text »
| PDF »
|
|