Response to Comment on Nishio et al. on Diabetes Reversal in NOD Mice
Junko Nishio,
Jason L. Gaglia,
Stuart E. Turvey,*
Christopher Campbell,
Christophe Benoist,
Diane Mathis
Contrary to previous findings, we found no significant differentiation of splenocytes into pancreatic islet cells in nonobese diabetic (NOD) mice treated with an immune adjuvant and allogenic spleen cells. We show that our single-nucleotide polymorphism assay has the requisite sensitivity to support our contention. The experiments of Faustman et al. lack adequate controls, and we maintain that no evidence of islet regeneration has been presented.
Section on Immunology and Immunogenetics, Joslin Diabetes Center, and Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, and Harvard Stem Cell Institute, 1 Joslin Place, Boston, MA 02215, USA.
* Present address: Division of Infectious and Immunological Diseases, Children's Hospital, Vancouver, British Columbia V6H 3V4, Canada.
To whom correspondence should be addressed. E-mail: cbdm{at}joslin.harvard.edu
