Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Science Signaling - Call For Papers

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 3 November 2006:
Vol. 314. no. 5800, pp. 818 - 821
DOI: 10.1126/science.1134027
Prev | Table of Contents | Next

Reports

Protrudin Induces Neurite Formation by Directional Membrane Trafficking

Michiko Shirane and Keiichi I. Nakayama*

Guanosine triphosphatases of the Rab family are key regulators of membrane trafficking, with Rab11 playing a specific role in membrane recycling. We identified a mammalian protein, protrudin, that promoted neurite formation through interaction with the guanosine diphosphate (GDP)–bound form of Rab11. Phosphorylation of protrudin by extracellular signal–regulated kinase (ERK) in response to nerve growth factor promoted protrudin association with Rab11-GDP. Down-regulation of protrudin by RNA interference induced membrane extension in all directions and inhibited neurite formation. Thus, protrudin regulates Rab11-dependent membrane recycling to promote the directional membrane trafficking required for neurite formation.

Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan. Precursory Research for Embryonic Science and Technology and Core Research for Evolutional Science and Technology, Japan Science and Technology Agency (JST), Kawaguchi, Saitama 332-0012, Japan.

* To whom correspondence should be addressed. E-mail: nakayak1{at}bioreg.kyushu-u.ac.jp

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The GDP-dependent Rab27a effector coronin 3 controls endocytosis of secretory membrane in insulin-secreting cell lines.
T. Kimura, Y. Kaneko, S. Yamada, H. Ishihara, T. Senda, A. Iwamatsu, and I. Niki (2008)
J. Cell Sci. 121, 3092-3098
   Abstract »    Full Text »    PDF »
Genetic testing for the spastic paraplegias: Drowning by numbers.
A. Durr (2008)
Neurology 71, 236-238
   Full Text »    PDF »
Receptor Type Protein Tyrosine Phosphatase {zeta}-Pleiotrophin Signaling Controls Endocytic Trafficking of DNER That Regulates Neuritogenesis.
N. Fukazawa, S. Yokoyama, M. Eiraku, M. Kengaku, and N. Maeda (2008)
Mol. Cell. Biol. 28, 4494-4506
   Abstract »    Full Text »    PDF »
Regulation of apoptosis and neurite extension by FKBP38 is required for neural tube formation in the mouse..
M. Shirane, M. Ogawa, J. Motoyama, and K. I. Nakayama (2008)
Genes Cells 13, 635-651
   Abstract »    Full Text »    PDF »
Anchoring of the 26S proteasome to the organellar membrane by FKBP38.
T. Nakagawa, M. Shirane, S.-i. Iemura, T. Natsume, and K. I. Nakayama (2007)
Genes Cells 12, 709-719
   Abstract »    Full Text »    PDF »
Wiring the nervous system: from form to function.
F. Matsuzaki and K. Sampath (2007)
Development 134, 1819-1822
   Abstract »    Full Text »    PDF »
Functional Symmetry of Endomembranes.
J. Saraste and B. Goud (2007)
Mol. Biol. Cell 18, 1430-1436
   Abstract »    Full Text »    PDF »
Phosphorylation of Protein Phosphatase 1 by Cyclin-dependent Protein Kinase 5 during Nerve Growth Factor-induced PC12 Cell Differentiation.
T. Li, L. E. Chalifour, and H. K. Paudel (2007)
J. Biol. Chem. 282, 6619-6628
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)