Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 27 October 2006:
Vol. 314. no. 5799, pp. 647 - 649
DOI: 10.1126/science.1124128
Prev | Table of Contents | Next

Reports

From the Genome to the Proteome: Uncovering Peptides in the Apis Brain

Amanda B. Hummon,1 Timothy A. Richmond,1 Peter Verleyen,2 Geert Baggerman,2 Jurgen Huybrechts,2 Michael A. Ewing,1 Evy Vierstraete,2 Sandra L. Rodriguez-Zas,3,4,5* Liliane Schoofs,2* Gene E. Robinson,4,5,6,7* Jonathan V. Sweedler1,4,5,7*

Neuropeptides, critical brain peptides that modulate animal behavior by affecting the activity of almost every neuronal circuit, are inherently difficult to predict directly from a nascent genome sequence because of extensive posttranslational processing. The combination of bioinformatics and proteomics allows unprecedented neuropeptide discovery from an unannotated genome. Within the Apis mellifera genome, we have inferred more than 200 neuropeptides and have confirmed the sequences of 100 peptides. This study lays the groundwork for future molecular studies of Apis neuropeptides with the identification of 36 genes, 33 of which were previously unreported.

1 Department of Chemistry, University of Illinois, Urbana, IL 61801, USA.
3 Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USA.
4 Institute for Genomic Biology, University of Illinois, Urbana, IL 61801, USA.
5 Neuroscience Program, University of Illinois, Urbana, IL 61801, USA.
6 Department of Entomology, University of Illinois, Urbana, IL 61801, USA.
2 Laboratory of Developmental Physiology, Genomics and Proteomics, Katholieke Universiteit Leuven, Naamsestraat 59, Louvain B-3000, Belgium.
7 Beckman Institute, University of Illinois, Urbana, IL 61801, USA.

* To whom correspondence should be addressed: jsweedle{at}uiuc.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Insulin signaling is involved in the regulation of worker division of labor in honey bee colonies.
S. A. Ament, M. Corona, H. S. Pollock, and G. E. Robinson (2008)
PNAS 105, 4226-4231
   Abstract »    Full Text »    PDF »
Prediction of neuropeptide cleavage sites in insects.
B. R. Southey, J. V. Sweedler, and S. L. Rodriguez-Zas (2008)
Bioinformatics 24, 815-825
   Abstract »    Full Text »    PDF »
Genomics, transcriptomics, and peptidomics of neuropeptides and protein hormones in the red flour beetle Tribolium castaneum.
B. Li, R. Predel, S. Neupert, F. Hauser, Y. Tanaka, G. Cazzamali, M. Williamson, Y. Arakane, P. Verleyen, L. Schoofs, et al. (2008)
Genome Res. 18, 113-122
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)