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Science 20 October 2006:
Vol. 314. no. 5798, pp. 464 - 467
DOI: 10.1126/science.1133348
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Reports

Dynamic Nuclear Actin Assembly by Arp2/3 Complex and a Baculovirus WASP-Like Protein

Erin D. Goley,1 Taro Ohkawa,2 Joel Mancuso,1 Jeffrey B. Woodruff,1 Joseph A. D'Alessio,1 W. Zacheus Cande,1 Loy E. Volkman,2 Matthew D. Welch1*

Diverse bacterial and viral pathogens induce actin polymerization in the cytoplasm of host cells to facilitate infection. Here, we describe a pathogenic mechanism for promoting dynamic actin assembly in the nucleus to enable viral replication. The baculovirus Autographa californica multiple nucleopolyhedrovirus induced nuclear actin polymerization by translocating the host actin-nucleating Arp2/3 complex into the nucleus, where it was activated by p78/83, a viral Wiskott-Aldrich syndrome protein (WASP)–like protein. Nuclear actin assembly by p78/83 and Arp2/3 complex was essential for viral progeny production. Recompartmentalizing dynamic host actin may represent a conserved mode of pathogenesis and reflect viral manipulation of normal functions of nuclear actin.

1 Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
2 Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA.

* To whom correspondence should be addressed. E-mail: welch{at}berkeley.edu

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Actin Polymerization Overshoots and ATP Hydrolysis as Assayed by Pyrene Fluorescence.
F. J. Brooks and A. E. Carlsson (2008)
Biophys. J. 95, 1050-1062
   Abstract »    Full Text »    PDF »
Autographa californica Multiple Nucleopolyhedrovirus Nucleocapsid Protein BV/ODV-C42 Mediates the Nuclear Entry of P78/83.
Y. Wang, Q. Wang, C. Liang, J. Song, N. Li, H. Shi, and X. Chen (2008)
J. Virol. 82, 4554-4561
   Abstract »    Full Text »    PDF »
Identification of a Hydrophobic Domain of HA2 Essential to Morphogenesis of Helicoverpa armigera Nucleopolyhedrovirus.
Q. Wang, Y. Wang, C. Liang, J. Song, and X. Chen (2008)
J. Virol. 82, 4072-4081
   Abstract »    Full Text »    PDF »


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