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Activity- and mTOR-Dependent Suppression of Kv1.1 Channel mRNA Translation in Dendrites
Kimberly F. Raab-Graham,*Patrick C. G. Haddick,*Yuh Nung Jan,Lily Yeh Jan
Mammalian target of rapamycin (mTOR) is implicated in synapticplasticity and local translation in dendrites. We found thatthe mTOR inhibitor, rapamycin, increased the Kv1.1 voltage-gatedpotassium channel protein in hippocampal neurons and promotedKv1.1 surface expression on dendrites without altering its axonalexpression. Moreover, endogenous Kv1.1 mRNA was detected indendrites. Using Kv1.1 fused to the photoconvertible fluorescenceprotein Kaede as a reporter for local synthesis, we observedKv1.1 synthesis in dendrites upon inhibition of mTOR or theN-methyl-D-aspartate (NMDA) glutamate receptor. Thus, synapticexcitation may cause local suppression of dendritic Kv1 channelsby reducing their local synthesis.
Howard Hughes Medical Institute, Departments of Physiology and Biochemistry, University of California, San Francisco, CA 94158, USA.
* These authors contributed equally to this work.
To whom correspondence should be addressed. E-mail: lily.jan{at}ucsf.edu
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