Modulation of Cell Adhesion and Motility in the Immune System by Myo1f

doi:10.1126/science.1131920

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Science 6 October 2006:
Vol. 314. no. 5796, pp. 136 - 139
DOI: 10.1126/science.1131920

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Modulation of Cell Adhesion and Motility in the Immune System by Myo1f

Sangwon V. Kim,¹^* Wajahat Z. Mehal,¹^,3 Xuemei Dong,⁶ Volkmar Heinrich,⁷ Marc Pypaert,⁴^,5 Ira Mellman,⁴^,5 Micah Dembo,⁷ Mark S. Mooseker,⁴^,8 Dianqing Wu,⁶ Richard A. Flavell¹^,2

Although class I myosins are known to play a wide range of roles,the physiological function of long-tailed class I myosins invertebrates remains elusive. We demonstrated that one of theseproteins, Myo1f, is expressed predominantly in the mammalianimmune system. Cells from Myo1f-deficient mice exhibited abnormallyincreased adhesion and reduced motility, resulting from augmentedexocytosis of ß2 integrin–containing granules.Also, the cortical actin that co-localizes with Myo1f was reducedin Myo1f-deficient cells. In vivo, Myo1f-deficient mice showedincreased susceptibility to infection by Listeria monocytogenesand an impaired neutrophil response. Thus, Myo1f directs immunecell motility and innate host defense against infection.

¹ Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA.
² Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06520, USA.
³ Section of Digestive Diseases, Yale University School of Medicine, New Haven, CT 06520, USA.
⁴ Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA.
⁵ Ludwig Institute for Cancer Research, Yale University School of Medicine, New Haven, CT 06520, USA.
⁶ Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT 06030, USA.
⁷ Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA.
⁸ Departments of Molecular, Cellular and Developmental Biology, and Department of Pathology, Yale University, New Haven, CT 06511, USA.

^* Present address: Immunology Program, Memorial Sloan-KetteringCancer Center, New York, NY 10021, USA.

Present address: Department of Biomedical Engineering, Universityof California Davis, Davis, CA 95616, USA.

Present address: Vascular Biology and Transplantation Programand Department of Pharmacology, Yale University School of Medicine,New Haven, CT 06520, USA.

To whom correspondence should be addressed. E-mail: richard.flavell{at}yale.edu

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