Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Transient infection of eukaryotic cells with commensal and extraintestinalpathogenic Escherichia coli of phylogenetic group B2 blocksmitosis and induces megalocytosis. This trait is linked to awidely spread genomic island that encodes giant modular nonribosomalpeptide and polyketide synthases. Contact with E. coli expressingthis gene cluster causes DNA double-strand breaks and activationof the DNA damage checkpoint pathway, leading to cell cyclearrest and eventually to cell death. Discovery of hybrid peptide-polyketidegenotoxins in E. coli will change our view on pathogenesis andcommensalism and open new biotechnological applications.
1 INRA, UMR1225, Ecole Nationale Vétérinaire de Toulouse, Toulouse F-31076, France. 2 Institut für Molekulare Infektionsbiologie, Universität Würzburg, Würzburg D-97070, Germany. 3 Göttingen Genomics Laboratory, Universität Göttingen, Göttingen D-37077, Germany. 4 Institut Pasteur, Unité de Génomique des Microorganismes Pathogènes and CNRS URA 2171, Paris F-75724, France.
* To whom correspondence should be addressed. E-mail: e.oswald{at}envt.fr
The editors suggest the following Related Resources on Science sites:
In Science Magazine
PERSPECTIVES
Tetsuya Hayashi (11 August 2006) Science313 (5788), 772.
[DOI: 10.1126/science.1131752] |Summary »|Full Text »|PDF »
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Structures of the Arm-type Binding Domains of HPI and HAI7 Integrases.
A. Szwagierczak, U. Antonenka, G. M. Popowicz, T. Sitar, T. A. Holak, and A. Rakin (2009)
J. Biol. Chem.
284, 31664-31671
|Abstract »|Full Text »|PDF »
Genetic Structure and Distribution of the Colibactin Genomic Island among Members of the Family Enterobacteriaceae.
J. Putze, C. Hennequin, J.-P. Nougayrede, W. Zhang, S. Homburg, H. Karch, M.-A. Bringer, C. Fayolle, E. Carniel, W. Rabsch, et al. (2009)
Infect. Immun.
77, 4696-4703
|Abstract »|Full Text »|PDF »
A bacterial type III effector family uses the papain-like hydrolytic activity to arrest the host cell cycle.
Q. Yao, J. Cui, Y. Zhu, G. Wang, L. Hu, C. Long, R. Cao, X. Liu, N. Huang, S. Chen, et al. (2009)
PNAS
106, 3716-3721
|Abstract »|Full Text »|PDF »
Cytolethal Distending Toxin Type I and Type IV Genes Are Framed with Lambdoid Prophage Genes in Extraintestinal Pathogenic Escherichia coli.
I. Toth, J.-P. Nougayrede, U. Dobrindt, T. N. Ledger, M. Boury, S. Morabito, T. Fujiwara, M. Sugai, J. Hacker, and E. Oswald (2009)
Infect. Immun.
77, 492-500
|Abstract »|Full Text »|PDF »
Enterococcus faecalis Induces Aneuploidy and Tetraploidy in Colonic Epithelial Cells through a Bystander Effect.
X. Wang, T. D. Allen, R. J. May, S. Lightfoot, C. W. Houchen, and M. M. Huycke (2008)
Cancer Res.
68, 9909-9917
|Abstract »|Full Text »|PDF »
Molecular Epidemiology and Phylogenetic Distribution of the Escherichia coli pks Genomic Island.
J. R. Johnson, B. Johnston, M. A. Kuskowski, J.-P. Nougayrede, and E. Oswald (2008)
J. Clin. Microbiol.
46, 3906-3911
|Abstract »|Full Text »|PDF »
Mycoplasma pneumoniae Infection Induces Reactive Oxygen Species and DNA Damage in A549 Human Lung Carcinoma Cells.
G. Sun, X. Xu, Y. Wang, X. Shen, Z. Chen, and J. Yang (2008)
Infect. Immun.
76, 4405-4413
|Abstract »|Full Text »|PDF »
Dichotomous metabolism of Enterococcus faecalis induced by haematin starvation modulates colonic gene expression.
T. D. Allen, D. R. Moore, X. Wang, V. Casu, R. May, M. R. Lerner, C. Houchen, D. J. Brackett, and M. M. Huycke (2008)
J. Med. Microbiol.
57, 1193-1204
|Abstract »|Full Text »|PDF »
Loss of Virulence Genes in Escherichia coli Populations during Manure Storage on a Commercial Swine Farm.
P. Duriez, Y. Zhang, Z. Lu, A. Scott, and E. Topp (2008)
Appl. Envir. Microbiol.
74, 3935-3942
|Abstract »|Full Text »|PDF »
The Klebsiella pheV tRNA locus: a hotspot for integration of alien genomic islands.
Molecular Basis of Commensalism in the Urinary Tract: Low Virulence or Virulence Attenuation?.
J. Zdziarski, C. Svanborg, B. Wullt, J. Hacker, and U. Dobrindt (2008)
Infect. Immun.
76, 695-703
|Abstract »|Full Text »|PDF »
Extraintestinal Virulence Is a Coincidental By-Product of Commensalism in B2 Phylogenetic Group Escherichia coli Strains.
T. Le Gall, O. Clermont, S. Gouriou, B. Picard, X. Nassif, E. Denamur, and O. Tenaillon (2007)
Mol. Biol. Evol.
24, 2373-2384
|Abstract »|Full Text »|PDF »
In Vivo Gene Expression Analysis Identifies Genes Required for Enhanced Colonization of the Mouse Urinary Tract by Uropathogenic Escherichia coli Strain CFT073 dsdA.
B. J. Haugen, S. Pellett, P. Redford, H. L. Hamilton, P. L. Roesch, and R. A. Welch (2007)
Infect. Immun.
75, 278-289
|Abstract »|Full Text »|PDF »