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Regulation of Sexual Development of Plasmodium by Translational Repression
Gunnar R. Mair,1Joanna A. M. Braks,1Lindsey S. Garver,2Joop C. A. G. Wiegant,3Neil Hall,4Roeland W. Dirks,3Shahid M. Khan,1George Dimopoulos,2Chris J. Janse,1Andrew P. Waters1*
Translational repression of messenger RNAs (mRNAs) plays animportant role in sexual differentiation and gametogenesis inmulticellular eukaryotes. Translational repression and mRNAturnover were shown to influence stage-specific gene expressionin the protozoan Plasmodium. The DDX6-class RNA helicase, DOZI(development of zygote inhibited), is found in a complex withmRNA species in cytoplasmic bodies of female, blood-stage gametocytes.These translationally repressed complexes are normally storedfor translation after fertilization. Genetic disruption of pbdoziinhibits the formation of the ribonucleoprotein complexes, andinstead, at least 370 transcripts are diverted to a degradationpathway.
1 Department of Parasitology, Leiden University Medical Centre, 2333 ZA Leiden, Netherlands. 2 Department of Molecular Microbiology and Immunology, Johns Hopkins School of Public Health, 615 North Wolfe Street, Baltimore, MD 21205, USA. 3 Department of Molecular Cell Biology, Leiden University Medical Centre, 2333 ZA Leiden, Netherlands. 4 The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
* To whom correspondence should be addressed. E-mail: waters{at}lumc.nl
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[DOI: 10.1126/science.1131299] |Summary »|Full Text »|PDF »
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