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Activated Signal Transduction Kinases Frequently Occupy Target Genes
Dmitry K. Pokholok,1*Julia Zeitlinger,1*Nancy M. Hannett,1David B. Reynolds,1Richard A. Young1,2
Cellular signal transduction pathways modify gene expressionprograms in response to changes in the environment, but themechanisms by which these pathways regulate populations of genesunder their control are not entirely understood. We presentevidence that most mitogen-activated protein kinases and proteinkinase A subunits become physically associated with the genesthat they regulate in the yeast (Saccharomyces cerevisiae) genome.The ability to detect this interaction of signaling kinaseswith target genes can be used to more precisely and comprehensivelymap the regulatory circuitry that eukaryotic cells use to respondto their environment.
1 Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA. 2 Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
* These authors contributed equally to this work.
To whom correspondence should be addressed. E-mail: young{at}wi.mit.edu
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To whom correspondence should be addressed. E-mail: 
