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Originally published in Science Express on 15 June 2006
Science 21 July 2006:
Vol. 313. no. 5785, pp. 357 - 360
DOI: 10.1126/science.1126953
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Reports

Structure of the Vesicular Stomatitis Virus Nucleoprotein-RNA Complex

Todd J. Green, Xin Zhang, Gail W. Wertz,* Ming Luo{dagger}

Vesicular stomatitis virus is a negative-stranded RNA virus. Its nucleoprotein (N) binds the viral genomic RNA and is involved in multiple functions including transcription, replication, and assembly. We have determined a 2.9 angstrom structure of a complex containing 10 molecules of the N protein and 90 bases of RNA. The RNA is tightly sequestered in a cavity at the interface between two lobes of the N protein. This serves to protect the RNA in the absence of polynucleotide synthesis. For the RNA to be accessed, some conformational change in the N protein should be necessary.

Department of Microbiology, School of Medicine, University of Alabama at Birmingham, 1025 18th Street South, Birmingham, AL 35294, USA.

* Present address: Department of Pathology, University of Virginia, MR5 Building Room 3051, Box 800904, 415 Lane Road, Charlottesville, VA 22908, USA.

{dagger} To whom correspondence should be addressed. E-mail: mingluo{at}uab.edu

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Science. ISSN 0036-8075 (print), 1095-9203 (online)