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Science 21 July 2006:
Vol. 313. no. 5785, pp. 347 - 351
DOI: 10.1126/science.1129551
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Reports

Receptor Activation Alters Inner Surface Potential During Phagocytosis

Tony Yeung,1,3 Mauricio Terebiznik,2 Liming Yu,1 John Silvius,4 Wasif M. Abidi,5 Mark Philips,5 Tim Levine,6 Andras Kapus,7 Sergio Grinstein1,3*

The surface potential of biological membranes varies according to their lipid composition. We devised genetically encoded probes to assess surface potential in intact cells. These probes revealed marked, localized alterations in the charge of the inner surface of the plasma membrane of macrophages during the course of phagocytosis. Hydrolysis of phosphoinositides and displacement of phosphatidylserine accounted for the change in surface potential at the phagosomal cup. Signaling molecules such as K-Ras, Rac1, and c-Src that are targeted to the membrane by electrostatic interactions were rapidly released from membrane subdomains where the surface charge was altered by lipid remodeling during phagocytosis.

1 Division of Cell Biology, Hepatology, and Nutrition Department, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada.
2 Gastroenterology, Hepatology, and Nutrition Department, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada.
3 Institute of Medical Sciences, University of Toronto, Toronto, Ontario M5S 1A8, Canada.
4 Department of Biochemistry, McGill University, Montreal, Quebec H3G 1Y6, Canada.
5 Department of Medicine, New York University School of Medicine, New York, NY 10016, USA.
6 Division of Cell Biology, University College, London EC1V 9EL, UK.
7 St. Michael's Hospital Research Institute, Toronto, Ontario M5B 1W8, Canada.

* To whom correspondence should be addressed. E-mail: sga{at}sickkids.ca

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Science. ISSN 0036-8075 (print), 1095-9203 (online)