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Receptor Activation Alters Inner Surface Potential During Phagocytosis
Tony Yeung,1,3Mauricio Terebiznik,2Liming Yu,1John Silvius,4Wasif M. Abidi,5Mark Philips,5Tim Levine,6Andras Kapus,7Sergio Grinstein1,3*
The surface potential of biological membranes varies accordingto their lipid composition. We devised genetically encoded probesto assess surface potential in intact cells. These probes revealedmarked, localized alterations in the charge of the inner surfaceof the plasma membrane of macrophages during the course of phagocytosis.Hydrolysis of phosphoinositides and displacement of phosphatidylserineaccounted for the change in surface potential at the phagosomalcup. Signaling molecules such as K-Ras, Rac1, and c-Src thatare targeted to the membrane by electrostatic interactions wererapidly released from membrane subdomains where the surfacecharge was altered by lipid remodeling during phagocytosis.
1 Division of Cell Biology, Hepatology, and Nutrition Department, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada. 2 Gastroenterology, Hepatology, and Nutrition Department, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada. 3 Institute of Medical Sciences, University of Toronto, Toronto, Ontario M5S 1A8, Canada. 4 Department of Biochemistry, McGill University, Montreal, Quebec H3G 1Y6, Canada. 5 Department of Medicine, New York University School of Medicine, New York, NY 10016, USA. 6 Division of Cell Biology, University College, London EC1V 9EL, UK. 7 St. Michael's Hospital Research Institute, Toronto, Ontario M5B 1W8, Canada.
* To whom correspondence should be addressed. E-mail: sga{at}sickkids.ca
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