Cell-Directed Assembly of Lipid-Silica Nanostructures Providing Extended Cell Viability
Helen K. Baca,1
Carlee Ashley,1
Eric Carnes,1
Deanna Lopez,1
Jeb Flemming,2
Darren Dunphy,2
Seema Singh,2
Zhu Chen,1
Nanguo Liu,3
Hongyou Fan,2
Gabriel P. López,1
Susan M. Brozik,2
Margaret Werner-Washburne,4
C. Jeffrey Brinker1,2,5*
Amphiphilic phospholipids were used to direct the formation
of biocompatible, uniform silica nanostructures in the presence
of
Saccharomyces cerevisiae and bacterial cell lines. The cell
surfaces organize multilayered phospholipid vesicles that interface
coherently with the silica host and help relieve drying stresses
that develop with conventional templates. These host structures
maintain cell accessibility, addressability, and viability in
the absence of buffer or an external fluidic architecture. The
cell surfaces are accessible and can be used to localize added
proteins, plasmids, and nanocrystals. Prolonged cell viability
combined with reporter protein expression enabled stand-alone
cell-based sensing.
2 Sandia National Laboratories, Albuquerque, NM 87185, USA.
3 Los Alamos National Laboratory, Chemistry Division, Los Alamos, NM 87545, USA.
4 Department of Biology, University of New Mexico, Albuquerque, NM 87131, USA.
5 Department of Molecular Genetics and Microbiology, University of New Mexico, Albuquerque, NM 87131, USA.
* To whom correspondence should be addressed. E-mail: cjbrink{at}sandia.gov
