Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Phire Hot Start DNA Polymerase

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 14 July 2006:
Vol. 313. no. 5784, pp. 187 - 191
DOI: 10.1126/science.1127683
Prev | Table of Contents | Next

Research Articles

Crystal Structure of the Low-pH Form of the Vesicular Stomatitis Virus Glycoprotein G

Stéphane Roche,* Stéphane Bressanelli,* Félix A. Rey,{dagger} Yves Gaudin{ddagger}

The vesicular stomatitis virus has an atypical membrane fusion glycoprotein (G) exhibiting a pH-dependent equilibrium between two forms at the virus surface. Membrane fusion is triggered during the transition from the high- to low-pH form. The structure of G in its low-pH form shows the classic hairpin conformation observed in all other fusion proteins in their postfusion conformation, in spite of a novel fold combining features of fusion proteins from classes I and II. The structure provides a framework for understanding the reversibility of the G conformational change. Unexpectedly, G is homologous to gB of herpesviruses, which raises important questions on viral evolution.

CNRS, Unité Mixte de Recherche (UMR) 2472, Institut Fédératif de Recherche (IFR) 115, Virologie Moléculaire et Structurale, 91198, Gif sur Yvette, France; Institut National de la Recherche Agronomique (INRA), UMR1157, Virologie Moléculaire et Structurale, 91198, Gif sur Yvette, France.

* These authors contributed equally to this work.

{dagger} Present address: Département de Virologie, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris cedex 15, France.

{ddagger} To whom correspondence should be addressed. E-mail: gaudin{at}vms.cnrs-gif.fr

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Glycoprotein B switches conformation during murid herpesvirus 4 entry.
L. Gillet, S. Colaco, and P. G. Stevenson (2008)
J. Gen. Virol. 89, 1352-1363
   Abstract »    Full Text »    PDF »
Aromatic Amino Acids in the Juxtamembrane Domain of Severe Acute Respiratory Syndrome Coronavirus Spike Glycoprotein Are Important for Receptor-Dependent Virus Entry and Cell-Cell Fusion.
M. W. Howard, E. A. Travanty, S. A. Jeffers, M. K. Smith, S. T. Wennier, L. B. Thackray, and K. V. Holmes (2008)
J. Virol. 82, 2883-2894
   Abstract »    Full Text »    PDF »
Molecular Architecture of the Bipartite Fusion Loops of Vesicular Stomatitis Virus Glycoprotein G, a Class III Viral Fusion Protein.
X. Sun, S. Belouzard, and G. R. Whittaker (2008)
J. Biol. Chem. 283, 6418-6427
   Abstract »    Full Text »    PDF »
The P2 capsid protein of the nonenveloped rice dwarf phytoreovirus induces membrane fusion in insect host cells.
F. Zhou, Y. Pu, T. Wei, H. Liu, W. Deng, C. Wei, B. Ding, T. Omura, and Y. Li (2007)
PNAS 104, 19547-19552
   Abstract »    Full Text »    PDF »
Bimolecular complementation reveals that glycoproteins gB and gH/gL of herpes simplex virus interact with each other during cell fusion.
D. Atanasiu, J. C. Whitbeck, T. M. Cairns, B. Reilly, G. H. Cohen, and R. J. Eisenberg (2007)
PNAS 104, 18718-18723
   Abstract »    Full Text »    PDF »
Hydrophobic Residues That Form Putative Fusion Loops of Epstein-Barr Virus Glycoprotein B Are Critical for Fusion Activity.
M. Backovic, T. S. Jardetzky, and R. Longnecker (2007)
J. Virol. 81, 9596-9600
   Abstract »    Full Text »    PDF »
Characterization of Fusion Determinants Points to the Involvement of Three Discrete Regions of Both E1 and E2 Glycoproteins in the Membrane Fusion Process of Hepatitis C Virus.
D. Lavillette, E.-I. Pecheur, P. Donot, J. Fresquet, J. Molle, R. Corbau, M. Dreux, F. Penin, and F.-L. Cosset (2007)
J. Virol. 81, 8752-8765
   Abstract »    Full Text »    PDF »
Random linker-insertion mutagenesis to identify functional domains of herpes simplex virus type 1 glycoprotein B.
E. Lin and P. G. Spear (2007)
PNAS 104, 13140-13145
   Abstract »    Full Text »    PDF »
Infectious Bursal Disease Virus, a Non-enveloped Virus, Possesses a Capsid-associated Peptide That Deforms and Perforates Biological Membranes.
M. Galloux, S. Libersou/, N. Morellet, S. Bouaziz, B. Da Costa, M. Ouldali, J. Lepault, and B. Delmas (2007)
J. Biol. Chem. 282, 20774-20784
   Abstract »    Full Text »    PDF »
CTX-BLAST: context sensitive version of protein BLAST.
A. Gambin and P. Wojtalewicz (2007)
Bioinformatics 23, 1686-1688
   Abstract »    Full Text »    PDF »
N-Terminal Mutants of Herpes Simplex Virus Type 2 gH Are Transported without gL but Require gL for Function.
T. M. Cairns, L. S. Friedman, H. Lou, J. C. Whitbeck, M. S. Shaner, G. H. Cohen, and R. J. Eisenberg (2007)
J. Virol. 81, 5102-5111
   Abstract »    Full Text »    PDF »
Mutational Evidence of Internal Fusion Loops in Herpes Simplex Virus Glycoprotein B.
B. P. Hannah, E. E. Heldwein, F. C. Bender, G. H. Cohen, and R. J. Eisenberg (2007)
J. Virol. 81, 4858-4865
   Abstract »    Full Text »    PDF »
Antigenic and Mutational Analyses of Herpes Simplex Virus Glycoprotein B Reveal Four Functional Regions.
F. C. Bender, M. Samanta, E. E. Heldwein, M. P. de Leon, E. Bilman, H. Lou, J. C. Whitbeck, R. J. Eisenberg, and G. H. Cohen (2007)
J. Virol. 81, 3827-3841
   Abstract »    Full Text »    PDF »
The Reovirus {sigma}1 Aspartic Acid Sandwich: A TRIMERIZATION MOTIF POISED FOR CONFORMATIONAL CHANGE.
P. Schelling, K. M. Guglielmi, E. Kirchner, B. Paetzold, T. S. Dermody, and T. Stehle (2007)
J. Biol. Chem. 282, 11582-11589
   Abstract »    Full Text »    PDF »
Thiol/Disulfide Exchange Is Required for Membrane Fusion Directed by the Newcastle Disease Virus Fusion Protein.
S. Jain, L. W. McGinnes, and T. G. Morrison (2007)
J. Virol. 81, 2328-2339
   Abstract »    Full Text »    PDF »
Structure of the Prefusion Form of the Vesicular Stomatitis Virus Glycoprotein G.
S. Roche, F. A. Rey, Y. Gaudin, and S. Bressanelli (2007)
Science 315, 843-848
   Abstract »    Full Text »    PDF »
Fusion of microglia with pyramidal neurons after retroviral infection..
J. B. Ackman, F. Siddiqi, R. S. Walikonis, and J. J. LoTurco (2006)
J. Neurosci. 26, 11413-11422
   Abstract »    Full Text »    PDF »
Membranes of the world unite!.
L. V. Chernomordik, J. Zimmerberg, and M. M. Kozlov (2006)
J. Cell Biol. 175, 201-207
   Abstract »    Full Text »    PDF »
Crystal structure of glycoprotein B from herpes simplex virus 1..
E. E. Heldwein, H. Lou, F. C. Bender, G. H. Cohen, R. J. Eisenberg, and S. C. Harrison (2006)
Science 313, 217-220
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)