Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
GoGreen Membership

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 7 July 2006:
Vol. 313. no. 5783, pp. 94 - 97
DOI: 10.1126/science.1128635
Prev | Table of Contents | Next

Reports

Prion-Induced Amyloid Heart Disease with High Blood Infectivity in Transgenic Mice

Matthew J. Trifilo,1 Toshitaka Yajima,2 Yusu Gu,2 Nancy Dalton,2 Kirk L. Peterson,2 Richard E. Race,3 Kimberly Meade-White,3 John L. Portis,3 Eliezer Masliah,4 Kirk U. Knowlton,2* Bruce Chesebro,3* Michael B. A. Oldstone1*

We investigated extraneural manifestations in scrapie-infected transgenic mice expressing prion protein lacking the glycophosphatydylinositol membrane anchor. In the brain, blood, and heart, both abnormal protease-resistant prion protein (PrPres) and prion infectivity were readily detected by immunoblot and by inoculation into nontransgenic recipients. The titer of infectious scrapie in blood plasma exceeded 107 50% infectious doses per milliliter. The hearts of these transgenic mice contained PrPres-positive amyloid deposits that led to myocardial stiffness and cardiac disease.

1 Viral-Immunobiology Laboratory, Departments of Molecular and Integrative Neurosciences and Infectology, Scripps Research Institute, La Jolla, CA 92037, USA.
2 Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
3 Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, MT 59840, USA.
4 Departments of Neurosciences and Pathology, University of California, San Diego, CA 92093, USA.

* To whom correspondence should be addressed. E-mail: mbaobo{at}scripps.edu (M.B.A.O.), bchesebro{at}niaid.nih.gov (B.C.), kknowlton{at}ucsd.edu (K.U.K.)

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Potential Role of the Ubiquitin-Proteasome System in Atherosclerosis: Aspects of a Protein Quality Disease.
J. Herrmann, S. M. Soares, L. O. Lerman, and A. Lerman (2008)
J. Am. Coll. Cardiol. 51, 2003-2010
   Abstract »    Full Text »    PDF »
Transmissible spongiform encephalopathy in the 21st century: Neuroscience for the clinical neurologist.
P. Brown (2008)
Neurology 70, 713-722
   Full Text »    PDF »
The Bitter End: The Ubiquitin-Proteasome System and Cardiac Dysfunction.
C. Patterson, C. Ike, P. W. Willis IV, G. A. Stouffer, and M. S. Willis (2007)
Circulation 115, 1456-1463
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)