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Originally published in Science Express on 18 May 2006
Science 23 June 2006:
Vol. 312. no. 5781, pp. 1791 - 1795
DOI: 10.1126/science.1127981
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Reports
The Structure of an Infectious P22 Virion Shows the Signal for Headful DNA Packaging
Gabriel C. Lander,1,2
Liang Tang,1
Sherwood R. Casjens,3
Eddie B. Gilcrease,3
Peter Prevelige,4
Anton Poliakov,4
Clinton S. Potter,2
Bridget Carragher,2
John E. Johnson1*
Bacteriophages, herpesviruses, and other large double-stranded DNA (dsDNA) viruses contain molecular machines that pump DNA into preassembled procapsids, generating internal capsid pressures exceeding, by 10-fold, that of bottled champagne. A 17 angstrom resolution asymmetric reconstruction of the infectious P22 virion reveals that tightly spooled DNA about the portal dodecamer forces a conformation that is significantly different from that observed in isolated portals assembled from ectopically expressed protein. We propose that the tight dsDNA spooling activates the switch that signals the headful chromosome packing density to the particle exterior.
1 Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
2 National Resource for Automated Molecular Microscopy, Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
3 Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT 84112, USA.
4 Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
* To whom correspondence should be addressed. E-mail: jackj{at}scripps.edu
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