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Extrafollicular Activation of Lymph Node B Cells by Antigen-Bearing Dendritic Cells
Hai Qi,Jackson G. Egen,Alex Y. C. Huang,Ronald N. Germain*
In contrast to naïve T cells that recognize short antigen-derivedpeptides displayed by specialized antigen-presenting cells,immunoglobulin receptors of B lymphocytes primarily recognizeintact proteins. How and where within a lymph node such unprocessedantigens become available for naïve B cell recognitionis not clear. We used two-photon intravital imaging to showthat, after exiting high-endothelial venules and before entryinto lymph node follicles, B cells survey locally concentrateddendritic cells. Engagement of the B cell receptor by the dendriticcell (DC)–associated antigen leads to lymphocyte calciumsignaling, migration arrest, antigen acquisition, and extrafollicularaccumulation. These findings suggest a possible role for antigen-specificB-DC interactions in promoting T cell–dependent antibodyresponses in vivo.
Lymphocyte Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD 20892, USA.
* To whom correspondence should be addressed. E-mail: rgermain{at}nih.gov
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