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Visualization of Cellulose Synthase Demonstrates Functional Association with Microtubules
Alexander R. Paredez,1,2Christopher R. Somerville,1,2David W. Ehrhardt2*
Expression of a functional yellow fluorescent protein fusionto cellulose synthase (CESA) in transgenic Arabidopsis plantsallowed the process of cellulose deposition to be visualizedin living cells. Spinning disk confocal microscopy revealedthat CESA complexes in the plasma membrane moved at constantrates in linear tracks that were aligned and were coincidentwith cortical microtubules. Within each observed linear track,complex movement was bidirectional. Inhibition of microtubulepolymerization changed the fine-scale distribution and patternof moving CESA complexes in the membrane, indicating a relativelydirect mechanism for guidance of cellulose deposition by thecytoskeleton.
1 Department of Biological Sciences, Stanford University, 260 Panama Street, Stanford, CA 94305, USA. 2 Carnegie Institution, Department of Plant Biology, 260 Panama Street, Stanford, CA 94305, USA.
* To whom correspondence should be addressed. E-mail: ehrhardt{at}stanford.edu
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