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CRACM1 Is a Plasma Membrane Protein Essential for Store-Operated Ca2+ Entry
M. Vig,1*C. Peinelt,2A. Beck,2D. L. Koomoa,2D. Rabah,1M. Koblan-Huberson,1S. Kraft,1H. Turner,2A. Fleig,2R. Penner,2*J.-P. Kinet1*
Store-operated Ca2+ entry is mediated by Ca2+ releaseactivatedCa2+ (CRAC) channels following Ca2+ release from intracellularstores. We performed a genome-wide RNA interference (RNAi) screenin Drosophila cells to identify proteins that inhibit store-operatedCa2+ influx. A secondary patch-clamp screen identified CRACM1and CRACM2 (CRAC modulators 1 and 2) as modulators of DrosophilaCRAC currents. We characterized the human ortholog of CRACM1,a plasma membraneresident protein encoded by gene FLJ14466.Although overexpression of CRACM1 did not affect CRAC currents,RNAi-mediated knockdown disrupted its activation. CRACM1 couldbe the CRAC channel itself, a subunit of it, or a componentof the CRAC signaling machinery.
1 Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA. 2 Center for Biomedical Research at The Queen's Medical Center and John A. Burns School of Medicine at the University of Hawaii, Honolulu, HI 96813, USA.
* To whom correspondence should be addressed. E-mail: mvig{at}bidmc.harvard.edu (M.V.); rpenner{at}hawaii.edu (R.P.); jkinet{at}bidmc.harvard.edu (J.-P.K.)
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Science Signaling
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