Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Originally published in Science Express on 11 May 2006
Science 26 May 2006:
Vol. 312. no. 5777, pp. 1218 - 1220
DOI: 10.1126/science.1122847
Prev | Table of Contents | Next

Reports

AXR4 Is Required for Localization of the Auxin Influx Facilitator AUX1

S. Dharmasiri,1*{dagger} R. Swarup,2* K. Mockaitis,1* N. Dharmasiri,1{dagger} S. K. Singh,3 M. Kowalchyk,3 A. Marchant,3 S. Mills,4 G. Sandberg,3 M. J. Bennett,2{ddagger} M. Estelle1{ddagger}

The AUX1 and PIN auxin influx and efflux facilitators are key regulators of root growth and development. For root gravitropism to occur, AUX1 and PIN2 must transport auxin via the lateral root cap to elongating epidermal cells. Genetic studies suggest that AXR4 functions in the same pathway as AUX1. Here we show that AXR4 is a previously unidentified accessory protein of the endoplasmic reticulum (ER) that regulates localization of AUX1 but not of PIN proteins. Loss of AXR4 resulted in abnormal accumulation of AUX1 in the ER of epidermal cells, indicating that the axr4 agravitropic phenotype is caused by defective AUX1 trafficking in the root epidermis.

1 Department of Biology, Indiana University, Bloomington, IN 47405, USA.
2 School of Biosciences, University of Nottingham, Loughborough LE12 5RD, UK.
3 Umeå Plant Science Centre, SLU, Umeå, Sweden.
4 School of Computer Science and Information Technology, University of Nottingham, Nottingham NG7 2UH, UK.

* These authors contributed equally to this work.

{dagger} Present address: Department of Biology, Texas State University–San Marcos, San Marcos, TX 78666, USA.

{ddagger} To whom correspondence should be addressed. E-mail: malcolm.bennett{at}nottingham.ac.uk (M.J.B.), maestell{at}indiana.edu (M.E.)

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Post-transcriptional regulation of auxin transport proteins: cellular trafficking, protein phosphorylation, protein maturation, ubiquitination, and membrane composition.
B. Titapiwatanakun and A. S. Murphy (2009)
J. Exp. Bot. 60, 1093-1107
   Abstract »    Full Text »    PDF »
Cellular and Molecular Requirements for Polar PIN Targeting and Transcytosis in Plants.
J. Kleine-Vehn, L. Langowski, J. Wisniewska, P. Dhonukshe, P. B Brewer, and J. Friml (2008)
Mol Plant 1, 1056-1066
   Abstract »    Full Text »    PDF »
The Binding of Auxin to the Arabidopsis Auxin Influx Transporter AUX1.
D. J. Carrier, N. T. A. Bakar, R. Swarup, R. Callaghan, R. M. Napier, M. J. Bennett, and I. D. Kerr (2008)
Plant Physiology 148, 529-535
   Abstract »    Full Text »    PDF »
A new mutant of Arabidopsis disturbed in its roots, right-handed slanting, and gravitropism defines a gene that encodes a heat-shock factor.
A. Fortunati, S. Piconese, P. Tassone, S. Ferrari, and F. Migliaccio (2008)
J. Exp. Bot.
   Abstract »    Full Text »    PDF »
Genetic Dissection of Hormonal Responses in the Roots of Arabidopsis Grown under Continuous Mechanical Impedance.
T. Okamoto, S. Tsurumi, K. Shibasaki, Y. Obana, H. Takaji, Y. Oono, and A. Rahman (2008)
Plant Physiology 146, 1651-1662
   Abstract »    Full Text »    PDF »
The Root Cap Determines Ethylene-Dependent Growth and Development in Maize Roots.
A. Hahn, R. Zimmermann, D. Wanke, K. Harter, and H. G. Edelmann (2008)
Mol Plant 1, 359-367
   Abstract »    Full Text »    PDF »
Functional Characterization of PaLAX1, a Putative Auxin Permease, in Heterologous Plant Systems.
K. Hoyerova, L. Perry, P. Hand, M. Lankova, T. Kocabek, S. May, J. Kottova, J. Paces, R. Napier, and E. Zazimalova (2008)
Plant Physiology 146, 1128-1141
   Abstract »    Full Text »    PDF »
Regulation of Root Nitrate Uptake at the NRT2.1 Protein Level in Arabidopsis thaliana.
J. Wirth, F. Chopin, V. Santoni, G. Viennois, P. Tillard, A. Krapp, L. Lejay, F. Daniel-Vedele, and A. Gojon (2007)
J. Biol. Chem. 282, 23541-23552
   Abstract »    Full Text »    PDF »
Ethylene Upregulates Auxin Biosynthesis in Arabidopsis Seedlings to Enhance Inhibition of Root Cell Elongation.
R. Swarup, P. Perry, D. Hagenbeek, D. Van Der Straeten, G. T.S. Beemster, G. Sandberg, R. Bhalerao, K. Ljung, and M. J. Bennett (2007)
PLANT CELL 19, 2186-2196
   Abstract »    Full Text »    PDF »
Subcellular Trafficking of the Arabidopsis Auxin Influx Carrier AUX1 Uses a Novel Pathway Distinct from PIN1.
J. Kleine-Vehn, P. Dhonukshe, R. Swarup, M. Bennett, and J. Friml (2006)
PLANT CELL 18, 3171-3181
   Abstract »    Full Text »    PDF »
Cytokinin-Mediated Cell Cycling Arrest of Pericycle Founder Cells in Lateral Root Initiation of Arabidopsis.
X. Li, X. Mo, H. Shou, and P. Wu (2006)
Plant Cell Physiol. 47, 1112-1123
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)