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Neuronal excitotoxicity during stroke is caused by activationof unidentified large-conductance channels, leading to swellingand calcium dysregulation. We show that ischemic-like conditions[O2/glucose deprivation (OGD)] open hemichannels, or half gapjunctions, in neurons. Hemichannel opening was indicated bya large linear current and flux across the membrane of smallfluorescent molecules. Single-channel openings of hemichannels(530 picosiemens) were observed in OGD. Both the current anddye flux were blocked by inhibitors of hemichannels. Therefore,hemichannel opening contributes to the profound ionic dysregulationduring stroke and may be a ubiquitous component of ischemicneuronal death.
Department of Psychiatry and Brain Research Centre, University of British Columbia, Vancouver, British Columbia V6T 2B5, Canada.
* To whom correspondence should be addressed. E-mail: bmacvica{at}interchange.ubc.ca
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