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Originally published in Science Express on 6 April 2006
Science 12 May 2006:
Vol. 312. no. 5775, p. 883
DOI: 10.1126/science.1121356
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Polar PIN Localization Directs Auxin Flow in Plants

Justyna Wisniewska,1,3 Jian Xu,2 Daniela Seifertová,1 Philip B. Brewer,1 Kamil Ruzicka,1 Ikram Blilou,2 David Rouquié,1* Eva Benková,1 Ben Scheres,2 Jirí Friml1{dagger}

Polar flow of the phytohormone auxin requires plasma membrane-associated PIN proteins and underlies multiple developmental processes in plants. Here we address the importance of the polarity of subcellular PIN localization for the directionality of auxin transport in Arabidopsis thaliana. Expression of different PINs in the root epidermis revealed the importance of PIN polar positions for directional auxin flow and root gravitropic growth. Interfering with sequence-embedded polarity signals directly demonstrates that PIN polarity is a primary factor in determining the direction of auxin flow in meristematic tissues. This finding provides a crucial piece in the puzzle of how auxin flow can be redirected via rapid changes in PIN polarity.

1 Center for Plant Molecular Biology (ZMBP), Tübingen University, D-72076 Tübingen, Germany.
2 Department of Molecular Genetics, Utrecht University, 3584CH Utrecht, Netherlands.
3 Department of Biotechnology, Institute of General and Molecular Biology, 87-100 Torun, Poland.

* Present address: Bayer CropScience, F-06560 Sophia Antipolis Cedex, France.

{dagger} To whom correspondence should be addressed. E-mail: jiri.friml{at}zmbp.uni-tuebingen.de

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