Related Content
Search Google Scholar for:
More Information
Related Jobs from ScienceCareers
|
Originally published in Science Express on 9 March 2006
Science 31 March 2006:
Vol. 311. no. 5769, pp. 1932 - 1936
DOI: 10.1126/science.1123726
|
|
Reports
Genome-Wide Detection of Polymorphisms at Nucleotide Resolution with a Single DNA Microarray
David Gresham,1,2*
Douglas M. Ruderfer,1,3
Stephen C. Pratt,1,3
Joseph Schacherer,1,3
Maitreya J. Dunham,1
David Botstein,1,2
Leonid Kruglyak1,3*
A central challenge of genomics is to detect, simply and inexpensively, all differences in sequence among the genomes of individual members of a species. We devised a system to detect all single-nucleotide differences between genomes with the use of data from a single hybridization to a whole-genome DNA microarray. This allowed us to detect a variety of spontaneous single–base pair substitutions, insertions, and deletions, and most (>90%) of the  30,000 known single-nucleotide polymorphisms between two Saccharomyces cerevisiae strains. We applied this approach to elucidate the genetic basis of phenotypic variants and to identify the small number of single–base pair changes accumulated during experimental evolution of yeast.
1 Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA.
2 Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
3 Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ 08544, USA.
* To whom correspondence should be addressed. E-mail: leonid{at}genomics.princeton.edu (L.K.); dgresham{at}genomics.princeton.edu (D.G.)
Read the Full Text
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
- Comparative Genomics of "Dehalococcoides ethenogenes" 195 and an Enrichment Culture Containing Unsequenced "Dehalococcoides" Strains.
- K. A. West, D. R. Johnson, P. Hu, T. Z. DeSantis, E. L. Brodie, P. K. H. Lee, H. Feil, G. L. Andersen, S. H. Zinder, and L. Alvarez-Cohen (2008)
Appl. Envir. Microbiol.
74, 3533-3540
| Abstract »
| Full Text »
| PDF »
- Empirical Evaluation of a New Method for Calculating Signal-to-Noise Ratio for Microarray Data Analysis.
- Z. He and J. Zhou (2008)
Appl. Envir. Microbiol.
74, 2957-2966
| Abstract »
| Full Text »
| PDF »
- Influence of genotype and nutrition on survival and metabolism of starving yeast.
- V. M. Boer, S. Amini, and D. Botstein (2008)
PNAS
105, 6930-6935
| Abstract »
| Full Text »
| PDF »
- Genomic Analysis of Adaptive Differentiation in Drosophila melanogaster.
- T. L. Turner, M. T. Levine, M. L. Eckert, and D. J. Begun (2008)
Genetics
179, 455-473
| Abstract »
| Full Text »
| PDF »
- Population genomics of the wild yeast Saccharomyces paradoxus: Quantifying the life cycle.
- I. J. Tsai, D. Bensasson, A. Burt, and V. Koufopanou (2008)
PNAS
105, 4957-4962
| Abstract »
| Full Text »
| PDF »
- Detecting genetic variation in microarray expression data.
- J. A. Greenhall, M. A. Zapala, M. Caceres, O. Libiger, C. Barlow, N. J. Schork, and D. J. Lockhart (2007)
Genome Res.
17, 1228-1235
| Abstract »
| Full Text »
| PDF »
- Genome sequencing and comparative analysis of Saccharomyces cerevisiae strain YJM789.
- W. Wei, J. H. McCusker, R. W. Hyman, T. Jones, Y. Ning, Z. Cao, Z. Gu, D. Bruno, M. Miranda, M. Nguyen, et al. (2007)
PNAS
104, 12825-12830
| Abstract »
| Full Text »
| PDF »
- Genome-wide identification of spliced introns using a tiling microarray.
- Z. Zhang, J. R. Hesselberth, and S. Fields (2007)
Genome Res.
17, 503-509
| Abstract »
| Full Text »
| PDF »
- Synthetic ecology: A model system for cooperation.
- M. J. Dunham (2007)
PNAS
104, 1741-1742
| Full Text »
| PDF »
- Genetic interaction screens advance in reverse.
- S. Komili and F. P. Roth (2007)
Genes & Dev.
21, 137-142
| Full Text »
| PDF »
- Transcript mapping with high-density oligonucleotide tiling arrays.
- W. Huber, J. Toedling, and L. M. Steinmetz (2006)
Bioinformatics
22, 1963-1970
| Abstract »
| Full Text »
| PDF »
- Mapping Novel Traits by Array-Assisted Bulk Segregant Analysis in Saccharomyces cerevisiae.
- M. J. Brauer, C. M. Christianson, D. A. Pai, and M. J. Dunham (2006)
Genetics
173, 1813-1816
| Abstract »
| Full Text »
| PDF »
|
|
