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Originally published in Science Express on 16 March 2006
Science 31 March 2006:
Vol. 311. no. 5769, pp. 1887 - 1893
DOI: 10.1126/science.1122771
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Research Articles

A Mitotic Lamin B Matrix Induced by RanGTP Required for Spindle Assembly

Ming-Ying Tsai,1 Shusheng Wang,1 Jill M. Heidinger,1,2 Dale K. Shumaker,3 Stephen A. Adam,3 Robert D. Goldman,3 Yixian Zheng1,2*

Mitotic spindle morphogenesis is a series of highly coordinated movements that lead to chromosome segregation and cytokinesis. We report that the intermediate filament protein lamin B, a component of the interphase nuclear lamina, functions in spindle assembly. Lamin B assembled into a matrix-like network in mitosis through a process that depended on the presence of the guanosine triphosphate–bound form of the small guanosine triphosphatase Ran. Depletion of lamin B resulted in defects in spindle assembly. Dominant negative mutant lamin B proteins that disrupt lamin B assembly in interphase nuclei also disrupted spindle assembly in mitosis. Furthermore, lamin B was essential for the formation of the mitotic matrix that tethers a number of spindle assembly factors. We propose that lamin B is a structural component of the long-sought-after spindle matrix that promotes microtubule assembly and organization in mitosis.

1 Department of Embryology, Carnegie Institution of Washington and Howard Hughes Medical Institute, Baltimore, MD 21218, USA.
2 Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA.
3 Department of Cell and Molecular Biology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.

* To whom correspondence should be addressed. E-mail: zheng{at}ciwemb.edu

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