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ReportsDesign and Evolution of New Catalytic Activity with an Existing Protein Scaffold![]()
The design of enzymes with new functions and properties has long been a goal in protein engineering. Here, we report a strategy to change the catalytic activity of an existing protein scaffold. This was achieved by simultaneous incorporation and adjustment of functional elements through insertion, deletion, and substitution of several active site loops, followed by point mutations to fine-tune the activity. Using this approach, we were able to introduce ß-lactamase activity into the
1 Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Kusung-Dong, Yusung-Gu, Daejon 305-701, Korea. * Present address: Nanormics Inc., Seoul, Korea.
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Science. ISSN 0036-8075 (print), 1095-9203 (online)