Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 13 January 2006:
Vol. 311. no. 5758, pp. 236 - 238
DOI: 10.1126/science.1115030
Prev | Table of Contents | Next

Reports

Long-Term Transmission of Defective RNA Viruses in Humans and Aedes Mosquitoes

John Aaskov,1 Katie Buzacott,1 Hlaing Myat Thu,1,2 Kym Lowry,1,3 Edward C. Holmes4*

In 2001, dengue virus type 1 (DENV-1) populations in humans and mosquitoes from Myanmar acquired a stop-codon mutation in the surface envelope (E) protein gene. Within a year, this stop-codon strain had spread to all individuals sampled. The presence of truncated E protein species within individual viral populations, along with a general relaxation in selective constraint, indicated that the stop-codon strain represents a defective lineage of DENV-1. We propose that such long-term transmission of defective RNA viruses in nature was achieved through complementation by coinfection of host cells with functional viruses.

1 School of Life Sciences, Queensland University of Technology, 2 George Street, Brisbane, 4001, Australia.
2 Department of Medical Research, Ziwaka Road, Yangon 11191, Myanmar.
3 Australian Army Malaria Institute, Enoggera, Brisbane, 4051, Australia.
4 Center for Infectious Disease Dynamics, Department of Biology, The Pennsylvania State University, Mueller Laboratory, University Park, PA 16802, USA.

* To whom correspondence should be addressed. E-mail: ech15{at}psu.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Effect of Ribavirin on the Mutation Rate and Spectrum of Hepatitis C Virus In Vivo.
J. M. Cuevas, F. Gonzalez-Candelas, A. Moya, and R. Sanjuan (2009)
J. Virol. 83, 5760-5764
   Abstract »    Full Text »    PDF »
Multiple-hit inhibition of infection by defective interfering particles.
K. A. Stauffer Thompson, G. A. Rempala, and J. Yin (2009)
J. Gen. Virol. 90, 888-899
   Abstract »    Full Text »    PDF »
Real-time PCR for detecting circulating dengue virus in the Guangdong Province of China in 2006.
Z. Bai, L. Liu, Z. Tu, L. Yao, J. Liu, B. Xu, B. Tang, J. Liu, Y. Wan, M. Fang, et al. (2008)
J. Med. Microbiol. 57, 1547-1552
   Abstract »    Full Text »    PDF »
Multiple recombinant dengue type 1 viruses in an isolate from a dengue patient.
J. Aaskov, K. Buzacott, E. Field, K. Lowry, A. Berlioz-Arthaud, and E. C. Holmes (2007)
J. Gen. Virol. 88, 3334-3340
   Abstract »    Full Text »    PDF »
Characterization of Hepatitis C Virus Deletion Mutants Circulating in Chronically Infected Patients.
S. Noppornpanth, S. L. Smits, T. X. Lien, Y. Poovorawan, A. D. M. E. Osterhaus, and B. L. Haagmans (2007)
J. Virol. 81, 12496-12503
   Abstract »    Full Text »    PDF »
Role of the mutant spectrum in adaptation and replication of West Nile virus.
A. T. Ciota, K. A. Ngo, A. O. Lovelace, A. F. Payne, Y. Zhou, P.-Y. Shi, and L. D. Kramer (2007)
J. Gen. Virol. 88, 865-874
   Abstract »    Full Text »    PDF »
Recent Advances in Deciphering Viral and Host Determinants of Dengue Virus Replication and Pathogenesis.
K. Clyde, J. L. Kyle, and E. Harris (2006)
J. Virol. 80, 11418-11431
   Full Text »    PDF »
Comparative analysis reveals no consistent association between the secondary structure of the 3'-untranslated region of dengue viruses and disease syndrome.
Y. Zhou, M. P. Mammen Jr, C. Klungthong, P. Chinnawirotpisan, D. W. Vaughn, S. Nimmannitya, S. Kalayanarooj, E. C. Holmes, and C. Zhang (2006)
J. Gen. Virol. 87, 2595-2603
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)