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Science 23 December 2005:
Vol. 310. no. 5756, pp. 1960 - 1963
DOI: 10.1126/science.1121925
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Reports

Protein Synthesis upon Acute Nutrient Restriction Relies on Proteasome Function

Ramunas M. Vabulas* and F. Ulrich Hartl*

The mechanisms that protect mammalian cells against amino acid deprivation are only partially understood. We found that during an acute decrease in external amino acid supply, before up-regulation of the autophagosomal-lysosomal pathway, efficient translation was ensured by proteasomal protein degradation. Amino acids for the synthesis of new proteins were supplied by the degradation of preexisting proteins, whereas nascent and newly formed polypeptides remained largely protected from proteolysis. Proteasome inhibition during nutrient deprivation caused rapid amino acid depletion and marked impairment of translation. Thus, the proteasome plays a crucial role in cell survival after acute disruption of amino acid supply.

Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.

* To whom correspondence should be addressed: E-mail: vabulas{at}biochem.mpg.de (R.M.V.); uhartl{at}biochem.mpg.de (F.U.H.)

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