Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 18 November 2005:
Vol. 310. no. 5751, pp. 1180 - 1183
DOI: 10.1126/science.1119409
Prev | Table of Contents | Next

Reports

Pre- and Postinvasion Defenses Both Contribute to Nonhost Resistance in Arabidopsis

Volker Lipka,1*{dagger} Jan Dittgen,1* Pawel Bednarek,1 Riyaz Bhat,1 Marcel Wiermer,1 Monica Stein,2 Jörn Landtag,3 Wolfgang Brandt,4 Sabine Rosahl,3 Dierk Scheel,3 Francisco Llorente,5 Antonio Molina,5 Jane Parker,1 Shauna Somerville,2 Paul Schulze-Lefert1{ddagger}

Nonhost resistance describes the immunity of an entire plant species against nonadapted pathogen species. We report that Arabidopsis PEN2 restricts pathogen entry of two ascomycete powdery mildew fungi that in nature colonize grass and pea species. The PEN2 glycosyl hydrolase localizes to peroxisomes and acts as a component of an inducible preinvasion resistance mechanism. Postinvasion fungal growth is blocked by a separate resistance layer requiring the EDS1-PAD4-SAG101 signaling complex, which is known to function in basal and resistance (R) gene–triggered immunity. Concurrent impairment of pre- and postinvasion resistance renders Arabidopsis a host for both nonadapted fungi.

1 Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl von Linné Weg 10, D-50829 Köln, Germany.
2 Department of Plant Biology, Carnegie Institute of Washington, Stanford, CA 94305, USA.
3 Department of Stress and Developmental Biology.
4 Department of Bioorganic Chemistry, Institute of Plant Biochemistry, Weinberg 3, D-06120 Halle (Saale), Germany.
5 Laboratorio de Bioquímica y Biología Molecular, Departamento de Biotecnología-Universidad Politécnica de Madrid Escuela Técnica Superior de Ingenieros Agrónomos, Avenida Complutense s/n, E-28040 Madrid, Spain.

* These authors contributed equally to this work.

{dagger} Present address: Zentrum für Molecularbiologie der Pflazen, Plant Biochemistry, Eberhard-Karls-Universität Tübingen, Auf der Morgenstelle 5, D-72076 Tübingen, Germany.

{ddagger} To whom correspondence should be addressed. E-mail: schlef{at}mpiz-koeln.mpg.de

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Arabidopsis HARMLESS TO OZONE LAYER Protein Methylates a Glucosinolate Breakdown Product and Functions in Resistance to Pseudomonas syringae pv. maculicola.
Y. Nagatoshi and T. Nakamura (2009)
J. Biol. Chem. 284, 19301-19309
   Abstract »    Full Text »    PDF »
Plant-Microbe Interactions: Chemical Diversity in Plant Defense.
P. Bednarek and A. Osbourn (2009)
Science 324, 746-748
   Abstract »    Full Text »    PDF »
In-Depth Proteome Analysis of Arabidopsis Leaf Peroxisomes Combined with in Vivo Subcellular Targeting Verification Indicates Novel Metabolic and Regulatory Functions of Peroxisomes.
S. Reumann, S. Quan, K. Aung, P. Yang, K. Manandhar-Shrestha, D. Holbrook, N. Linka, R. Switzenberg, C. G. Wilkerson, A. P.M. Weber, et al. (2009)
Plant Physiology 150, 125-143
   Abstract »    Full Text »    PDF »
Biotic and Abiotic Stimulation of Root Epidermal Cells Reveals Common and Specific Responses to Arbuscular Mycorrhizal Fungi.
A. Genre, G. Ortu, C. Bertoldo, E. Martino, and P. Bonfante (2009)
Plant Physiology 149, 1424-1434
   Abstract »    Full Text »    PDF »
Gene expression profiling and silencing reveal that monolignol biosynthesis plays a critical role in penetration defence in wheat against powdery mildew invasion.
N. H. Bhuiyan, G. Selvaraj, Y. Wei, and J. King (2009)
J. Exp. Bot. 60, 509-521
   Abstract »    Full Text »    PDF »
Glucosinolate Metabolites Required for an Arabidopsis Innate Immune Response.
N. K. Clay, A. M. Adio, C. Denoux, G. Jander, and F. M. Ausubel (2009)
Science 323, 95-101
   Abstract »    Full Text »    PDF »
A Glucosinolate Metabolism Pathway in Living Plant Cells Mediates Broad-Spectrum Antifungal Defense.
P. Bednarek, M. Pislewska-Bednarek, A. Svatos, B. Schneider, J. Doubsky, M. Mansurova, M. Humphry, C. Consonni, R. Panstruga, A. Sanchez-Vallet, et al. (2009)
Science 323, 101-106
   Abstract »    Full Text »    PDF »
Quantitative Proteomic Analysis of Bean Plants Infected by a Virulent and Avirulent Obligate Rust Fungus.
J. Lee, J. Feng, K. B. Campbell, B. E. Scheffler, W. M. Garrett, S. Thibivilliers, G. Stacey, D. Q. Naiman, M. L. Tucker, M. A. Pastor-Corrales, et al. (2009)
Mol. Cell. Proteomics 8, 19-31
   Abstract »    Full Text »    PDF »
Focal accumulation of defences at sites of fungal pathogen attack.
W. Underwood and S. C. Somerville (2008)
J. Exp. Bot. 59, 3501-3508
   Abstract »    Full Text »    PDF »
Secretory Pathways in Plant Immune Responses.
C. Kwon, P. Bednarek, and P. Schulze-Lefert (2008)
Plant Physiology 147, 1575-1583
   Full Text »    PDF »
Glycerol-3-Phosphate Levels Are Associated with Basal Resistance to the Hemibiotrophic Fungus Colletotrichum higginsianum in Arabidopsis.
B. Chanda, S. C. Venugopal, S. Kulshrestha, D. A. Navarre, B. Downie, L. Vaillancourt, A. Kachroo, and P. Kachroo (2008)
Plant Physiology 147, 2017-2029
   Abstract »    Full Text »    PDF »
Antagonistic Jacalin-Related Lectins Regulate the Size of ER Body-Type {beta}-Glucosidase Complexes in Arabidopsis thaliana.
A. J. Nagano, Y. Fukao, M. Fujiwara, M. Nishimura, and I. Hara-Nishimura (2008)
Plant Cell Physiol. 49, 969-980
   Abstract »    Full Text »    PDF »
A Genome-Wide Functional Investigation into the Roles of Receptor-Like Proteins in Arabidopsis.
G. Wang, U. Ellendorff, B. Kemp, J. W. Mansfield, A. Forsyth, K. Mitchell, K. Bastas, C.-M. Liu, A. Woods-Tor, C. Zipfel, et al. (2008)
Plant Physiology 147, 503-517
   Abstract »    Full Text »    PDF »
Mutation of a Gene in the Fungus Leptosphaeria maculans Allows Increased Frequency of Penetration of Stomatal Apertures of Arabidopsis thaliana.
C. E. Elliott, Harjono, and B. J. Howlett (2008)
Mol Plant
   Abstract »    Full Text »    PDF »
A Lesion-Mimic Syntaxin Double Mutant in Arabidopsis Reveals Novel Complexity of Pathogen Defense Signaling.
Z. Zhang, A. Lenk, M. X. Andersson, T. Gjetting, C. Pedersen, M. E. Nielsen, M.-A. Newman, B.-H. Hou, S. C. Somerville, and H. Thordal-Christensen (2008)
Mol Plant 1, 510-527
   Abstract »    Full Text »    PDF »
The coi1-16 Mutant Harbors a Second Site Mutation Rendering PEN2 Nonfunctional.
L. Westphal, D. Scheel, and S. Rosahl (2008)
PLANT CELL 20, 824-826
   Full Text »    PDF »
The Necrotroph Botrytis cinerea Induces a Non-Host Type II Resistance Mechanism in Pinus pinaster Suspension-Cultured Cells.
H. Azevedo, T. Lino-Neto, and R. M. Tavares (2008)
Plant Cell Physiol. 49, 386-395
   Abstract »    Full Text »    PDF »
Light Induces Peroxisome Proliferation in Arabidopsis Seedlings through the Photoreceptor Phytochrome A, the Transcription Factor HY5 HOMOLOG, and the Peroxisomal Protein PEROXIN11b.
M. Desai and J. Hu (2008)
Plant Physiology 146, 1117-1127
   Abstract »    Full Text »    PDF »
Powdery Mildew Induces Defense-Oriented Reprogramming of the Transcriptome in a Susceptible But Not in a Resistant Grapevine.
R. W.M. Fung, M. Gonzalo, C. Fekete, L. G. Kovacs, Y. He, E. Marsh, L. M. McIntyre, D. P. Schachtman, and W. Qiu (2008)
Plant Physiology 146, 236-249
   Abstract »    Full Text »    PDF »
Interaction between SGT1 and Cytosolic/Nuclear HSC70 Chaperones Regulates Arabidopsis Immune Responses.
L. D. Noel, G. Cagna, J. Stuttmann, L. Wirthmuller, S. Betsuyaku, C.-P. Witte, R. Bhat, N. Pochon, T. Colby, and J. E. Parker (2007)
PLANT CELL 19, 4061-4076
   Abstract »    Full Text »    PDF »
ARGONAUTE4 Is Required for Resistance to Pseudomonas syringae in Arabidopsis.
A. Agorio and P. Vera (2007)
PLANT CELL 19, 3778-3790
   Abstract »    Full Text »    PDF »
Proteome Analysis of Arabidopsis Leaf Peroxisomes Reveals Novel Targeting Peptides, Metabolic Pathways, and Defense Mechanisms.
S. Reumann, L. Babujee, C. Ma, S. Wienkoop, T. Siemsen, G. E. Antonicelli, N. Rasche, F. Luder, W. Weckwerth, and O. Jahn (2007)
PLANT CELL 19, 3170-3193
   Abstract »    Full Text »    PDF »
Quantitative Phosphoproteomics of Early Elicitor Signaling in Arabidopsis.
J. J. Benschop, S. Mohammed, M. O'Flaherty, A. J. R. Heck, M. Slijper, and F. L. H. Menke (2007)
Mol. Cell. Proteomics 6, 1198-1214
   Abstract »    Full Text »    PDF »
Barley MLO Modulates Actin-Dependent and Actin-Independent Antifungal Defense Pathways at the Cell Periphery.
M. Miklis, C. Consonni, R. A. Bhat, V. Lipka, P. Schulze-Lefert, and R. Panstruga (2007)
Plant Physiology 144, 1132-1143
   Abstract »    Full Text »    PDF »
ABA Is an Essential Signal for Plant Resistance to Pathogens Affecting JA Biosynthesis and the Activation of Defenses in Arabidopsis.
B. A.T. Adie, J. Perez-Perez, M. M. Perez-Perez, M. Godoy, J.-J. Sanchez-Serrano, E. A. Schmelz, and R. Solano (2007)
PLANT CELL 19, 1665-1681
   Abstract »    Full Text »    PDF »
Impairment of Cellulose Synthases Required for Arabidopsis Secondary Cell Wall Formation Enhances Disease Resistance.
C. Hernandez-Blanco, D. X. Feng, J. Hu, A. Sanchez-Vallet, L. Deslandes, F. Llorente, M. Berrocal-Lobo, H. Keller, X. Barlet, C. Sanchez-Rodriguez, et al. (2007)
PLANT CELL 19, 890-903
   Abstract »    Full Text »    PDF »
Targeted alterations in iron homeostasis underlie plant defense responses.
G. Liu, D. L. Greenshields, R. Sammynaiken, R. N. Hirji, G. Selvaraj, and Y. Wei (2007)
J. Cell Sci. 120, 596-605
   Abstract »    Full Text »    PDF »
The PEROXIN11 Protein Family Controls Peroxisome Proliferation in Arabidopsis.
T. Orth, S. Reumann, X. Zhang, J. Fan, D. Wenzel, S. Quan, and J. Hu (2007)
PLANT CELL 19, 333-350
   Abstract »    Full Text »    PDF »
Production of reactive oxygen species in Arabidopsis thaliana cell suspension cultures in response to an elicitor from Fusarium oxysporum: implications for basal resistance.
D. R Davies, L. V Bindschedler, T. S Strickland, and G P. Bolwell (2006)
J. Exp. Bot. 57, 1817-1827
   Abstract »    Full Text »    PDF »
Salicylic Acid-Independent ENHANCED DISEASE SUSCEPTIBILITY1 Signaling in Arabidopsis Immunity and Cell Death Is Regulated by the Monooxygenase FMO1 and the Nudix Hydrolase NUDT7.
M. Bartsch, E. Gobbato, P. Bednarek, S. Debey, J. L. Schultze, J. Bautor, and J. E. Parker (2006)
PLANT CELL 18, 1038-1051
   Abstract »    Full Text »    PDF »
Insights into nonhost disease resistance: can they assist disease control in agriculture?.
J. Ellis (2006)
PLANT CELL 18, 523-528
   Full Text »    PDF »
Loss of AtPDR8, a Plasma Membrane ABC Transporter of Arabidopsis thaliana, Causes Hypersensitive Cell Death Upon Pathogen Infection.
Y. Kobae, T. Sekino, H. Yoshioka, T. Nakagawa, E. Martinoia, and M. Maeshima (2006)
Plant Cell Physiol. 47, 309-318
   Abstract »    Full Text »    PDF »
Arabidopsis PEN3/PDR8, an ATP Binding Cassette Transporter, Contributes to Nonhost Resistance to Inappropriate Pathogens That Enter by Direct Penetration.
M. Stein, J. Dittgen, C. Sanchez-Rodriguez, B.-H. Hou, A. Molina, P. Schulze-Lefert, V. Lipka, and S. Somerville (2006)
PLANT CELL 18, 731-746
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)