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Science 21 October 2005:
Vol. 310. no. 5747, pp. 493 - 496
DOI: 10.1126/science.1118155
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Reports

Reciprocal Interference Between Specific CJD and Scrapie Agents in Neural Cell Cultures

Noriuki Nishida,1,2 Shigeru Katamine,3 Laura Manuelidis1*

Infection of mice with an attenuated Creutzfeldt-Jakob disease agent (SY-CJD) interferes with superinfection by a more virulent human-derived CJD agent (FU-CJD) and does not require pathological prion protein (PrPres). Using a rapid coculture system, we found that a neural cell line free of immune system cells similarly supported substantial CJD agent interference without PrPres. In addition, SY-CJD prevented superinfection by sheep-derived Chandler (Ch) and 22L scrapie agents. However, only 22L and not Ch prevented FU-CJD infection, even though both scrapie strains provoked abundant PrPres. This relationship between particular strains of sheep- and human-derived agents is likely to affect their prevalence and epidemic spread.

1 Yale Medical School, New Haven, CT 06510, USA.
2 Center for Emerging Infectious Diseases, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan.
3 Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Medicine, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.

* To whom correspondence should be addressed. E-mail: laura.manuelidis{at}yale.edu

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cells infected with scrapie and Creutzfeldt-Jakob disease agents produce intracellular 25-nm virus-like particles.
L. Manuelidis, Z.-X. Yu, N. Barquero, and B. Mullins (2007)
PNAS 104, 1965-1970
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Prion Interference Is Due to a Reduction in Strain-Specific PrPSc Levels.
J. C. Bartz, M. L. Kramer, M. H. Sheehan, J. A. L. Hutter, J. I. Ayers, R. A. Bessen, and A. E. Kincaid (2007)
J. Virol. 81, 689-697
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Continuum of prion protein structures enciphers a multitude of prion isolate-specified phenotypes.
G. Legname, H.-O. B. Nguyen, D. Peretz, F. E. Cohen, S. J. DeArmond, and S. B. Prusiner (2006)
PNAS 103, 19105-19110
   Abstract »    Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)