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Arabidopsis H+-PPase AVP1 Regulates Auxin-Mediated Organ Development
Jisheng Li,1*Haibing Yang,1*Wendy Ann Peer,3Gregory Richter,2Joshua Blakeslee,3Anindita Bandyopadhyay,3Boosaree Titapiwantakun,3Soledad Undurraga,1Mariya Khodakovskaya,1Elizabeth L. Richards,3Beth Krizek,4Angus S. Murphy,3Simon Gilroy,2Roberto Gaxiola1
The transport of auxin controls developmental events in plants.Here, we report that in addition to maintaining vacuolar pH,the H+-pyrophosphatase, AVP1, controls auxin transport and consequentlyauxin-dependent development. AVP1 overexpression results inincreased cell division at the onset of organ formation, hyperplasia,and increased auxin transport. In contrast, avp1-1 null mutantshave severely disrupted root and shoot development and reducedauxin transport. Changes in the expression of AVP1 affect thedistribution and abundance of the P–adenosine triphosphataseand Pinformed 1 auxin efflux facilitator, two proteins implicatedin auxin distribution. Thus, AVP1 facilitates the auxin fluxesthat regulate organogenesis.
1 Department of Plant Science, University of Connecticut, Storrs, CT 06268, USA. 2 Biology Department, Pennsylvania State University, University Park, PA 16802, USA. 3 Horticulture Department, Purdue University, West Lafayette, IN 47907, USA. 4 Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA.
* These authors contributed equally to this work.
To whom correspondence should be addressed. E-mail: roberto.gaxiola{at}uconn.edu
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