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Originally published in Science Express on 4 August 2005
Science 2 September 2005:
Vol. 309. no. 5740, pp. 1573 - 1576
DOI: 10.1126/science.1115079
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Reports

Inhibition of Translational Initiation by Let-7 MicroRNA in Human Cells

Ramesh S. Pillai,1 Suvendra N. Bhattacharyya,1 Caroline G. Artus,1 Tabea Zoller,1 Nicolas Cougot,2 Eugenia Basyuk,2 Edouard Bertrand,2 Witold Filipowicz1*

MicroRNAs (miRNAs) are ~21-nucleotide-long RNA molecules regulating gene expression in multicellular eukaryotes. In metazoa, miRNAs act by imperfectly base-pairing with the 3' untranslated region of target messenger RNAs (mRNAs) and repressing protein accumulation by an unknown mechanism. We demonstrate that endogenous let-7 microribonucleoproteins (miRNPs) or the tethering of Argonaute (Ago) proteins to reporter mRNAs in human cells inhibit translation initiation. M7G-cap-independent translation is not subject to repression, suggesting that miRNPs interfere with recognition of the cap. Repressed mRNAs, Ago proteins, and miRNAs were all found to accumulate in processing bodies. We propose that localization of mRNAs to these structures is a consequence of translational repression.

1 Friedrich Miescher Institute for Biomedical Research, 4002 Basel, Switzerland.
2 Institut de Génétique Moléculaire de Montpellier, 34000 Montpellier, France.

* To whom correspondence should be addressed. E-mail: Filipowi{at}fmi.ch

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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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G. Stoecklin and P. Anderson (2007)
Genes & Dev. 21, 627-631
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TTP and BRF proteins nucleate processing body formation to silence mRNAs with AU-rich elements.
T. M. Franks and J. Lykke-Andersen (2007)
Genes & Dev. 21, 719-735
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2'-Fluoro-4'-thioarabino-modified oligonucleotides: conformational switches linked to siRNA activity.
J. K. Watts, N. Choubdar, K. Sadalapure, F. Robert, A. S. Wahba, J. Pelletier, B. Mario Pinto, and M. J. Damha (2007)
Nucleic Acids Res. 35, 1441-1451
   Abstract »    Full Text »    PDF »
Antiviral Protein APOBEC3G Localizes to Ribonucleoprotein Complexes Found in P Bodies and Stress Granules.
S. Gallois-Montbrun, B. Kramer, C. M. Swanson, H. Byers, S. Lynham, M. Ward, and M. H. Malim (2007)
J. Virol. 81, 2165-2178
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Dynamic regulation of miRNA expression in ordered stages of cellular development.
J. R. Neilson, G. X.Y. Zheng, C. B. Burge, and P. A. Sharp (2007)
Genes & Dev. 21, 578-589
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Identification and characterization of human Mex-3 proteins, a novel family of evolutionarily conserved RNA-binding proteins differentially localized to processing bodies.
K. Buchet-Poyau, J. Courchet, H. L. Hir, B. Seraphin, J.-Y. Scoazec, L. Duret, C. Domon-Dell, J.-N. Freund, and M. Billaud (2007)
Nucleic Acids Res. 35, 1289-1300
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