Related Content
Search Google Scholar for:
More Information
Related Jobs from ScienceCareers
|
|
Science 26 August 2005: Vol. 309. no. 5739, pp. 1369 - 1373 DOI: 10.1126/science.1116447
|
|
Reports
Nuclear Reprogramming of Somatic Cells After Fusion with Human Embryonic Stem Cells
Chad A. Cowan,
Jocelyn Atienza,
Douglas A. Melton,
Kevin Eggan*
We have explored the use of embryonic stem cells as an alternative to oocytes for reprogramming human somatic nuclei. Human embryonic stem (hES) cells were fused with human fibroblasts, resulting in hybrid cells that maintain a stable tetraploid DNA content and have morphology, growth rate, and antigen expression patterns characteristic of hES cells. Differentiation of hybrid cells in vitro and in vivo yielded cell types from each embryonic germ layer. Analysis of genome-wide transcriptional activity, reporter gene activation, allele-specific gene expression, and DNA methylation showed that the somatic genome was reprogrammed to an embryonic state. These results establish that hES cells can reprogram the transcriptional state of somatic nuclei and provide a system for investigating the underlying mechanisms.
Howard Hughes Medical Institute, Harvard Stem Cell Institute, Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA.
* To whom correspondence should be addressed. E-mail: Eggan{at}mcb.harvard.edu
Read the Full Text
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
- Efficient acquisition of dual metastasis organotropism to bone and lung through stable spontaneous fusion between MDA-MB-231 variants.
- X. Lu and Y. Kang (2009)
PNAS
106, 9385-9390
| Abstract »
| Full Text »
| PDF »
- Nuclear reprogramming in heterokaryons is rapid, extensive, and bidirectional.
- A. Palermo, R. Doyonnas, N. Bhutani, J. Pomerantz, O. Alkan, and H. M. Blau (2009)
FASEB J
23, 1431-1440
| Abstract »
| Full Text »
| PDF »
- Reprogramming to a muscle fate by fusion recapitulates differentiation.
- J. H. Pomerantz, S. Mukherjee, A. T. Palermo, and H. M. Blau (2009)
J. Cell Sci.
122, 1045-1053
| Abstract »
| Full Text »
| PDF »
- Change in gene expression of mouse embryonic stem cells derived from parthenogenetic activation.
- S. P. Gong, H. Kim, E. J. Lee, S. T. Lee, S. Moon, H.-J. Lee, and J. M. Lim (2009)
Hum. Reprod.
24, 805-814
| Abstract »
| Full Text »
| PDF »
- Systematic identification of cis-silenced genes by trans complementation.
- J. H. Lee, B. Bugarija, E. J. Millan, N. M. Walton, J. Gaetz, C. J. Fernandes, W.-H. Yu, N. Mekel-Bobrov, T. W. Vallender, G. E. Snyder, et al. (2009)
Hum. Mol. Genet.
18, 835-846
| Abstract »
| Full Text »
| PDF »
- Physical transfer of membrane and cytoplasmic components as a general mechanism of cell-cell communication.
- X. Niu, K. Gupta, J. T. Yang, M. J. Shamblott, and A. Levchenko (2009)
J. Cell Sci.
122, 600-610
| Abstract »
| Full Text »
| PDF »
- Epigenetic reprogramming and induced pluripotency.
- K. Hochedlinger and K. Plath (2009)
Development
136, 509-523
| Abstract »
| Full Text »
| PDF »
- Reversine Increases the Plasticity of Lineage-committed Cells toward Neuroectodermal Lineage.
- E. K. Lee, G.-U. Bae, J. S. You, J. C. Lee, Y. J. Jeon, J. W. Park, J. H. Park, S. H. Ahn, Y. K. Kim, W. S. Choi, et al. (2009)
J. Biol. Chem.
284, 2891-2901
| Abstract »
| Full Text »
| PDF »
- A big promise from the very small identification of circulating embryonic stem-like pluripotent cells in patients with acute myocardial infarction..
- D. W. Losordo and R. Kishore (2009)
J. Am. Coll. Cardiol.
53, 10-12
| Full Text »
| PDF »
- Esrrb Activates Oct4 Transcription and Sustains Self-renewal and Pluripotency in Embryonic Stem Cells.
- X. Zhang, J. Zhang, T. Wang, M. A. Esteban, and D. Pei (2008)
J. Biol. Chem.
283, 35825-35833
| Abstract »
| Full Text »
| PDF »
- The cytoplasm of mouse germinal vesicle stage oocytes can enhance somatic cell nuclear reprogramming.
- H.-T. Bui, S. Wakayama, S. Kishigami, J.-H. Kim, N. Van Thuan, and T. Wakayama (2008)
Development
135, 3935-3945
| Abstract »
| Full Text »
| PDF »
- Effects of Akt signaling on nuclear reprogramming.
- T. Nakamura, K. Inoue, S. Ogawa, H. Umehara, N. Ogonuki, H. Miki, T. Kimura, A. Ogura, and T. Nakano (2008)
Genes Cells
13, 1269-1277
| Abstract »
| Full Text »
| PDF »
- Induced Pluripotency of Mouse and Human Somatic Cells.
- N. Maherali and K. Hochedlinger (2008)
Cold Spring Harb Symp Quant Biol
| Abstract »
| PDF »
- Reprogramming of Somatic Cell Identity.
- J. Hanna, B.W. Carey, and R. Jaenisch (2008)
Cold Spring Harb Symp Quant Biol
| Abstract »
| PDF »
- Understanding pluripotency--how embryonic stem cells keep their options open.
- B.V. Johnson, N. Shindo, P.D. Rathjen, J. Rathjen, and R.A. Keough (2008)
Mol. Hum. Reprod.
14, 513-520
| Abstract »
| Full Text »
| PDF »
- Pluripotent stem cell lines.
- J. Yu and J. A. Thomson (2008)
Genes & Dev.
22, 1987-1997
| Abstract »
| Full Text »
| PDF »
- T-Cell Factor 3 Regulates Embryonic Stem Cell Pluripotency and Self-Renewal by the Transcriptional Control of Multiple Lineage Pathways.
- W.-L. Tam, C. Y. Lim, J. Han, J. Zhang, Y.-S. Ang, H.-H. Ng, H. Yang, and B. Lim (2008)
Stem Cells
26, 2019-2031
| Abstract »
| Full Text »
| PDF »
- G9a and Jhdm2a Regulate Embryonic Stem Cell Fusion-Induced Reprogramming of Adult Neural Stem Cells.
- D. K. Ma, C.-H. J. Chiang, K. Ponnusamy, G.-l. Ming, and H. Song (2008)
Stem Cells
26, 2131-2141
| Abstract »
| Full Text »
| PDF »
- Pluripotency and nuclear reprogramming.
- S. Yamanaka (2008)
Phil Trans R Soc B
363, 2079-2087
| Abstract »
| Full Text »
| PDF »
- Cell-Free Embryonic Stem Cell Extract-Mediated Derivation of Multipotent Stem Cells From NIH3T3 Fibroblasts for Functional and Anatomical Ischemic Tissue Repair.
- J. Rajasingh, E. Lambers, H. Hamada, E. Bord, T. Thorne, I. Goukassian, P. Krishnamurthy, K. M. Rosen, D. Ahluwalia, Y. Zhu, et al. (2008)
Circ. Res.
102, e107-e117
| Abstract »
| Full Text »
| PDF »
- What can we learn from gene expression profiling of mouse oocytes?.
- T. Hamatani, M. Yamada, H. Akutsu, N. Kuji, Y. Mochimaru, M. Takano, M. Toyoda, K. Miyado, A. Umezawa, and Y. Yoshimura (2008)
Reproduction
135, 581-592
| Abstract »
| Full Text »
| PDF »
- Generation of isogenic pluripotent stem cells.
- J. A. Byrne (2008)
Hum. Mol. Genet.
17, R37-R41
| Abstract »
| Full Text »
| PDF »
- Phenotype reversion in fetal human liver epithelial cells identifies the role of an intermediate meso-endodermal stage before hepatic maturation.
- M. Inada, A. Follenzi, K. Cheng, M. Surana, B. Joseph, D. Benten, S. Bandi, H. Qian, and S. Gupta (2008)
J. Cell Sci.
121, 1002-1013
| Abstract »
| Full Text »
| PDF »
- Epigenetic reprogramming in embryonic and foetal development upon somatic cell nuclear transfer cloning.
- H. Niemann, X C. Tian, W A. King, and R. S F Lee (2008)
Reproduction
135, 151-163
| Abstract »
| Full Text »
| PDF »
- Stem Cell Induced Regeneration of Myocardium.
- R. P. Gallegos and R. M. Bolman III (2008)
Card. Surg. Adult
3, 1657-1668
| Full Text »
- Review Article: Stem Cells in Human Reproduction.
- C. E. Gargett (2007)
Reproductive Sciences
14, 405-424
| Abstract »
| PDF »
- Reversine increases the plasticity of lineage-committed mammalian cells.
- S. Chen, S. Takanashi, Q. Zhang, W. Xiong, S. Zhu, E. C. Peters, S. Ding, and P. G. Schultz (2007)
PNAS
104, 10482-10487
| Abstract »
| Full Text »
| PDF »
- Checkpoint-apoptosis uncoupling in human and mouse embryonic stem cells: a source of karyotpic instability.
- C. Mantel, Y. Guo, M. R. Lee, M.-K. Kim, M.-K. Han, H. Shibayama, S. Fukuda, M. C. Yoder, L. M. Pelus, K.-S. Kim, et al. (2007)
Blood
109, 4518-4527
| Abstract »
| Full Text »
| PDF »
- Genome-Wide Reprogramming in Hybrids of Somatic Cells and Embryonic Stem Cells.
- D. J. Ambrosi, B. Tanasijevic, A. Kaur, C. Obergfell, R. J. O'Neill, W. Krueger, and T. P. Rasmussen (2007)
Stem Cells
25, 1104-1113
| Abstract »
| Full Text »
| PDF »
- Epigenetic Reprogramming of OCT4 and NANOG Regulatory Regions by Embryonal Carcinoma Cell Extract.
- C. T. Freberg, J. A. Dahl, S. Timoskainen, and P. Collas (2007)
Mol. Biol. Cell
18, 1543-1553
| Abstract »
| Full Text »
| PDF »
- Fusion of Human Hematopoietic Progenitor Cells and Murine Cardiomyocytes Is Mediated by {alpha}4{beta}1 Integrin/Vascular Cell Adhesion Molecule-1 Interaction.
- S. Zhang, E. Shpall, J. T. Willerson, and E. T.H. Yeh (2007)
Circ. Res.
100, 693-702
| Abstract »
| Full Text »
| PDF »
- Active tissue-specific DNA demethylation conferred by somatic cell nuclei in stable heterokaryons.
- F. Zhang, J. H. Pomerantz, G. Sen, A. T. Palermo, and H. M. Blau (2007)
PNAS
104, 4395-4400
| Abstract »
| Full Text »
| PDF »
- Foetal fibroblasts introduced to cleaving mouse embryos contribute to full-term development.
- A. Piliszek, J. A Modlinski, K. Pysniak, and J. Karasiewicz (2007)
Reproduction
133, 207-218
| Abstract »
| Full Text »
| PDF »
- Reprogramming somatic cells into stem cells..
- R. Alberio, K. H Campbell, and A. D Johnson (2006)
Reproduction
132, 709-720
| Abstract »
| Full Text »
| PDF »
- An ethical analysis of alternative methods to obtain pluripotent stem cells without destroying embryos.
- H. Mertes, G. Pennings, and A. Van Steirteghem (2006)
Hum. Reprod.
21, 2749-2755
| Abstract »
| Full Text »
| PDF »
- Concise Review: Scientific and Ethical Roadblocks to Human Embryonic Stem Cell Therapy.
- L. Gruen and L. Grabel (2006)
Stem Cells
24, 2162-2169
| Abstract »
| Full Text »
| PDF »
- The transcriptome of human oocytes.
- A. M. Kocabas, J. Crosby, P. J. Ross, H. H. Otu, Z. Beyhan, H. Can, W.-L. Tam, G. J. M. Rosa, R. G. Halgren, B. Lim, et al. (2006)
PNAS
103, 14027-14032
| Abstract »
| Full Text »
| PDF »
- Abnormal CpG island methylation occurs during in vitro differentiation of human embryonic stem cells.
- Y. Shen, J. Chow, Z. Wang, and G. Fan (2006)
Hum. Mol. Genet.
15, 2623-2635
| Abstract »
| Full Text »
| PDF »
- Human embryonic stem cells have a unique epigenetic signature.
- M. Bibikova, E. Chudin, B. Wu, L. Zhou, E. W. Garcia, Y. Liu, S. Shin, T. W. Plaia, J. M. Auerbach, D. E. Arking, et al. (2006)
Genome Res.
16, 1075-1083
| Abstract »
| Full Text »
| PDF »
- Using Therapeutic Cloning to Fight Human Disease: A Conundrum or Reality?.
- V. J. Hall, P. Stojkovic, and M. Stojkovic (2006)
Stem Cells
24, 1628-1637
| Abstract »
| Full Text »
| PDF »
- Cellular Memory and Hematopoietic Stem Cell Aging.
- L. M. Kamminga and G. de Haan (2006)
Stem Cells
24, 1143-1149
| Abstract »
| Full Text »
| PDF »
- The Production and Directed Differentiation of Human Embryonic Stem Cells.
- A. Trounson (2006)
Endocr. Rev.
27, 208-219
| Abstract »
| Full Text »
| PDF »
- Scientific and clinical opportunities for modeling blood disorders with embryonic stem cells.
- M. W. Lensch and G. Q. Daley (2006)
Blood
107, 2605-2612
| Abstract »
| Full Text »
| PDF »
- Chromatin Decondensation and Nuclear Reprogramming by Nucleoplasmin.
- H. Tamada, N. V. Thuan, P. Reed, D. Nelson, N. Katoku-Kikyo, J. Wudel, T. Wakayama, and N. Kikyo (2006)
Mol. Cell. Biol.
26, 1259-1271
| Abstract »
| Full Text »
| PDF »
- Induction of Dedifferentiation, Genomewide Transcriptional Programming, and Epigenetic Reprogramming by Extracts of Carcinoma and Embryonic Stem Cells.
- C. K. Taranger, A. Noer, A. L. Sorensen, A.-M. Hakelien, A. C. Boquest, and P. Collas (2005)
Mol. Biol. Cell
16, 5719-5735
| Abstract »
| Full Text »
| PDF »
- A primate virus generates transformed human cells by fusion.
- D. M. Duelli, S. Hearn, M. P. Myers, and Y. Lazebnik (2005)
J. Cell Biol.
171, 493-503
| Abstract »
| Full Text »
| PDF »
- A tetraploid twist on the embryonic stem cell..
- E. G. Phimister (2005)
N. Engl. J. Med.
353, 1646-1647
| Full Text »
| PDF »
|
|