Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Originally published in Science Express on 14 July 2005
Science 19 August 2005:
Vol. 309. no. 5738, pp. 1251 - 1253
DOI: 10.1126/science.1116006
Prev | Table of Contents | Next

Reports

Drosophila RNAi Screen Reveals CD36 Family Member Required for Mycobacterial Infection

Jennifer A. Philips,1* Eric J. Rubin,3 Norbert Perrimon1,2*

Certain pathogens, such as Mycobacterium tuberculosis, survive within the hostile intracellular environment of a macrophage. To identify host factors required for mycobacterial entry and survival within macrophages, we performed a genomewide RNA interference screen in Drosophila macrophage-like cells, using Mycobacterium fortuitum. We identified factors required for general phagocytosis, as well as those needed specifically for mycobacterial infection. One specific factor, Peste (Pes), is a CD36 family member required for uptake of mycobacteria, but not Escherichia coli or Staphylococcus aureus. Moreover, mammalian class B scavenger receptors (SRs) conferred uptake of bacteria into nonphagocytic cells, with SR-BI and SR-BII uniquely mediating uptake of M. fortuitum, which suggests a conserved role for class B SRs in pattern recognition and innate immunity.

1 Department of Genetics, Harvard Medical School, 77 Louis Pasteur Avenue, Boston, MA 02115, USA.
2 Howard Hughes Medical Institute, Harvard Medical School, 77 Louis Pasteur Avenue, Boston, MA 02115, USA.
3 Department of Immunology and Infectious Disease, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, USA.

* To whom correspondence should be addressed. E-mail: jphilips{at}partners.org (J.A.P.), perrimon{at}receptor.med.harvard.edu (N.P.)

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
CD36, a Scavenger Receptor Involved in Immunity, Metabolism, Angiogenesis, and Behavior.
R. L. Silverstein and M. Febbraio (2009)
Science Signaling 2, re3
   Abstract »    Full Text »    PDF »
Automated High-Throughput siRNA Transfection in Raw 264.7 Macrophages: A Case Study for Optimization Procedure.
J.-P. Carralot, T.-K. Kim, B. Lenseigne, A. S. Boese, P. Sommer, A. Genovesio, and P. Brodin (2009)
J Biomol Screen 14, 151-160
   Abstract »    PDF »
Role of Human CD36 in Bacterial Recognition, Phagocytosis, and Pathogen-Induced JNK-Mediated Signaling.
I. N. Baranova, R. Kurlander, A. V. Bocharov, T. G. Vishnyakova, Z. Chen, A. T. Remaley, G. Csako, A. P. Patterson, and T. L. Eggerman (2008)
J. Immunol. 181, 7147-7156
   Abstract »    Full Text »    PDF »
SNMP is a signaling component required for pheromone sensitivity in Drosophila.
X. Jin, T. S. Ha, and D. P. Smith (2008)
PNAS 105, 10996-11001
   Abstract »    Full Text »    PDF »
Scavenger Receptor Class B Is Required for Hepatitis C Virus Uptake and Cross-Presentation by Human Dendritic Cells.
H. Barth, E. K. Schnober, C. Neumann-Haefelin, C. Thumann, M. B. Zeisel, H. M. Diepolder, Z. Hu, T. J. Liang, H. E. Blum, R. Thimme, et al. (2008)
J. Virol. 82, 3466-3479
   Abstract »    Full Text »    PDF »
Use of Drosophila S2 Cells as a Model for Studying Ehrlichia chaffeensis Infections.
A. Luce-Fedrow, T. Von Ohlen, D. Boyle, R. R. Ganta, and S. K. Chapes (2008)
Appl. Envir. Microbiol. 74, 1886-1891
   Abstract »    Full Text »    PDF »
ESCRT factors restrict mycobacterial growth.
J. A. Philips, M. C. Porto, H. Wang, E. J. Rubin, and N. Perrimon (2008)
PNAS 105, 3070-3075
   Abstract »    Full Text »    PDF »
Role for lysosomal enzyme -hexosaminidase in the control of mycobacteria infection.
I. C. Koo, Y. M. Ohol, P. Wu, J. H. Morisaki, J. S. Cox, and E. J. Brown (2008)
PNAS 105, 710-715
   Abstract »    Full Text »    PDF »
Rac2 is a major actor of Drosophila resistance to Pseudomonas aeruginosa acting in phagocytic cells.
A. Avet-Rochex, J. Perrin, E. Bergeret, and M.-O. Fauvarque (2007)
Genes Cells 12, 1193-1204
   Abstract »    Full Text »    PDF »
Identification of Mycobacterium avium pathogenicity island important for macrophage and amoeba infection.
L. Danelishvili, M. Wu, B. Stang, M. Harriff, S. Cirillo, J. Cirillo, R. Bildfell, B. Arbogast, and L. E. Bermudez (2007)
PNAS 104, 11038-11043
   Abstract »    Full Text »    PDF »
Knockdown of Actin and Caspase Gene Expression by RNA Interference in the Symbiotic Anemone Aiptasia pallida.
S. R. Dunn, W. S. Phillips, D. R. Green, and V. M. Weis (2007)
Biol. Bull. 212, 250-258
   Abstract »    Full Text »    PDF »
Downregulation of CD4 by Human Immunodeficiency Virus Type 1 Nef Is Dependent on Clathrin and Involves Direct Interaction of Nef with the AP2 Clathrin Adaptor.
R. Chaudhuri, O. W. Lindwasser, W. J. Smith, J. H. Hurley, and J. S. Bonifacino (2007)
J. Virol. 81, 3877-3890
   Abstract »    Full Text »    PDF »
TLR2-Mediated Survival of Staphylococcus aureus in Macrophages: A Novel Bacterial Strategy against Host Innate Immunity.
I. Watanabe, M. Ichiki, A. Shiratsuchi, and Y. Nakanishi (2007)
J. Immunol. 178, 4917-4925
   Abstract »    Full Text »    PDF »
PhenomicDB: a new cross-species genotype/phenotype resource.
P. Groth, N. Pavlova, I. Kalev, S. Tonov, G. Georgiev, H.-D. Pohlenz, and B. Weiss (2007)
Nucleic Acids Res. 35, D696-D699
   Abstract »    Full Text »    PDF »
Applications of High-Throughput RNA Interference Screens to Problems in Cell and Developmental Biology.
N. Perrimon and B. Mathey-Prevot (2007)
Genetics 175, 7-16
   Abstract »    Full Text »    PDF »
Induced Expression of Pattern Recognition Receptors in Cumulus Oocyte Complexes: Novel Evidence for Innate Immune-Like Functions during Ovulation.
M. Shimada, I. Hernandez-Gonzalez, I. Gonzalez-Robanya, and J. S. Richards (2006)
Mol. Endocrinol. 20, 3228-3239
   Abstract »    Full Text »    PDF »
CLA-1 and its splicing variant CLA-2 mediate bacterial adhesion and cytosolic bacterial invasion in mammalian cells.
T. G. Vishnyakova, R. Kurlander, A. V. Bocharov, I. N. Baranova, Z. Chen, M. S. Abu-Asab, M. Tsokos, D. Malide, F. Basso, A. Remaley, et al. (2006)
PNAS 103, 16888-16893
   Abstract »    Full Text »    PDF »
Genome-wide functional analysis of human cell-cycle regulators.
M. Mukherji, R. Bell, L. Supekova, Y. Wang, A. P. Orth, S. Batalov, L. Miraglia, D. Huesken, J. Lange, C. Martin, et al. (2006)
PNAS 103, 14819-14824
   Abstract »    Full Text »    PDF »
Hepatitis C virus entry: potential receptors and their biological functions..
L. Cocquerel, C. Voisset, and J. Dubuisson (2006)
J. Gen. Virol. 87, 1075-1084
   Abstract »    Full Text »    PDF »
Drosophila Genome-wide RNAi Screens: Are They Delivering the Promise?.
B. MATHEY-PREVOT and N. PERRIMON (2006)
Cold Spring Harb Symp Quant Biol 71, 141-148
   Abstract »    PDF »
Genome-Wide RNAi Screen for Host Factors Required for Intracellular Bacterial Infection.
H. Agaisse, L. S. Burrack, J. A. Philips, E. J. Rubin, N. Perrimon, and D. E. Higgins (2005)
Science 309, 1248-1251
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)