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Science 12 August 2005:
Vol. 309. no. 5737, pp. 1090 - 1092
DOI: 10.1126/science.1113109
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Reports

Complete Genome Sequence and Lytic Phase Transcription Profile of a Coccolithovirus

William H. Wilson,1* Declan C. Schroeder,2 Michael J. Allen,1 Matthew T. G. Holden,3 Julian Parkhill,3 Bart G. Barrell,3 Carol Churcher,3 Nancy Hamlin,3 Karen Mungall,3 Halina Norbertczak,3 Michael A. Quail,3 Claire Price,3 Ester Rabbinowitsch,3 Danielle Walker,3 Marie Craigon,4 Douglas Roy,4 Peter Ghazal4

The genus Coccolithovirus is a recently discovered group of viruses that infect the globally important marine calcifying microalga Emiliania huxleyi. Among the 472 predicted genes of the 407,339–base pair genome are a variety of unexpected genes, most notably those involved in biosynthesis of ceramide, a sphingolipid known to induce apoptosis. Uniquely for algal viruses, it also contains six RNA polymerase subunits and a novel promoter, suggesting this virus encodes its own transcription machinery. Microarray transcriptomic analysis reveals that 65% of the predicted virus-encoded genes are expressed during lytic infection of E. huxleyi.

1 Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, PL1 3DH, UK.
2 Marine Biological Association, Citadel Hill, Plymouth, PL1 2PB, UK.
3 Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK.
4 Scottish Centre for Genomic Technology and Informatics, Chancellor's Building, College of Medicine, University of Edinburgh, 49 Little France Crescent, Edinburgh EH16 4SB, UK.

* To whom correspondence should be addressed. E-mail: whw{at}pml.ac.uk

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Science. ISSN 0036-8075 (print), 1095-9203 (online)