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Science 29 July 2005:
Vol. 309. no. 5735, pp. 771 - 774
DOI: 10.1126/science.1113988
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Reports

Organization of Iron-Sulfur Clusters in Respiratory Complex I

Philip Hinchliffe and Leonid A. Sazanov*

Complex I of respiratory chains plays a central role in bioenergetics and is implicated in many human neurodegenerative diseases. An understanding of its mechanism requires a knowledge of the organization of redox centers. The arrangement of iron-sulfur clusters in the hydrophilic domain of complex I from Thermus thermophilus has been determined with the use of x-ray crystallography. One binuclear and six tetranuclear clusters are arranged, maximally 14 angstroms apart, in an 84-angstrom-long electron transfer chain. The binuclear cluster N1a and the tetranuclear cluster N7 are not in this pathway. Cluster N1a may play a role in the prevention of oxidative damage. The structure provides a framework for the interpretation of the large amounts of data accumulated on complex I.

Medical Research Council (MRC) Dunn Human Nutrition Unit, Wellcome Trust/MRC Building, Hills Road, Cambridge CB2 2XY, UK.

* To whom correspondence should be addressed. E-mail: sazanov{at}mrc-dunn.cam.ac.uk

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Science. ISSN 0036-8075 (print), 1095-9203 (online)